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ABSTRACT: The defensive function of calcium oxalate crystals

PROVIDER: PRJNA657125 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12460603_1.fastq.gz	Fastqsanger.gz
	SRR12460603_2.fastq.gz	Fastqsanger.gz
	SRR12473529_1.fastq.gz	Fastqsanger.gz
	SRR12473529_2.fastq.gz	Fastqsanger.gz
	SRR12494544_1.fastq.gz	Fastqsanger.gz

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Analysis of altered microRNA expression profiles in proximal renal tubular cells in response to calcium oxalate monohydrate crystal adhesion

Project description:The present study aims to assess the potential changes in microRNAs of proximal renal tubular cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals. microRNA microarray was applied to evaluate the expression of HK-2 cells exposed to COM crystals for 0 and 24 hours.

2014-04-22 | E-GEOD-56934 | biostudies-arrayexpress

Analysis of altered microRNA expression profiles in proximal renal tubular cells in response to calcium oxalate monohydrate crystal adhesion

Project description:The present study aims to assess the potential changes in microRNAs of proximal renal tubular cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals.

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Calcium oxalate crystals with a bipyramidal morphology and a high tendency to aggregate attach directly to breast cancer cells and affect their phenotype

Project description:Various types of crystal can form in our bodies under normal and pathological conditions, with the more harmful of these crystals being studied intensively. However, much less is known about calcium oxalate dihydrate (COD) crystals, which are commonly associated with benign conditions. In general, calcium oxalates are pathological biominerals found in kidney stones and as microcalcifications (MCs) in breast and thyroid cancers. In breast cancer MCs, COD crystals are exclusively associated with benign lesions, and we have recently shown that they suppress breast precancer progression in vitro. Here, we studied the influence of synthetic calcium oxalate crystals – both COD and calcium oxalate monohydrate (COM) – with different morphologies and properties on crystal-cell interactions. We found that the less negatively charged crystals exhibited a greater tendency to aggregate and, as aggregates, attached better to cells, particularly to the cells of an invasive breast cancer cell line. We did not find any association between crystallinity, surface area, and solubilities of the crystals and attachment to cells. The crystal types that did attach efficiently to the cells, namely, COD with a thin or thick bipyramidal morphology, had the greatest impact on cell phenotype and functionally delayed cell growth, most probably through contact inhibition. Notably, in terms of morphology and size, these crystals resembled COD MCs found in benign breast tissues, suggesting that they are involved in a mechanism of tumor suppression.

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Analysis of altered LncRNA expression profiles in proximal renal tubular cells in response to calcium oxalate monohydrate crystal adhesion

Project description:The present study aims to assess the potential changes in LncRNAs of proximal renal cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals.

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Analysis of altered LncRNA expression profiles in proximal renal tubular cells in response to calcium oxalate monohydrate crystal adhesion

Project description:The present study aims to assess the potential changes in LncRNAs of proximal renal cells in response to the adhesion of calcium oxalate monohydrate (COM) crystals. lncRNA microarray were applied to evaluate the expression of HK-2 cells exposed to COM crystal for 0 and 24 hours.

2014-04-26 | E-GEOD-57111 | biostudies-arrayexpress

Effect of calcium oxalate microcrystals on kidney proximal tubule epithelial cell gene expression in microgravity

Project description:We evaluated the impact of spaceflight on a microphysiologic model of calcium oxalate (CaOx) kidney stone disease. Proximal tubule epithelial cells cultured as confluent microtubules were exposed to CaOx crystals with or without potassium citrate (a potential countermeasure) to determine impact on gene expression. Nine genes were differentially expressed in response to CaOx crystal exposure during spaceflight. This project presents the use of microgravity as a unique environment to study kidney pathophysiology.

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Project description:ADPKD is the most common genetic renal disease, leading to renal failure in the vast majority of affected individuals. Standard therapy consists of blood pressure control and salt restriction. Tolvaptan further delays renal failure but side effects prevent its widespread use. Novel therapeutic approaches are needed. Recent evidence has identified inflammation as an important driver of ADPKD. Here we show a strong inflammatory signature in transcriptomes of early ADPKD in the mouse. IL1β and IL18, effectors of inflammasome activation, are among the top upregulated transcripts. Inflammasomes act as part of the innate immune response to danger signals, including crystals. Induction of calcium oxalate crystals through dietary intervention in Pkd1 mutant mice aggravated the cystic phenotype. Treatment of Pkd1 mutant mice with the inflammasome inhibitor MCC950 significantly ameliorated the ADPKD phenotype. Analogous results were obtained through genetic silencing of the essential inflammasome component Asc in ADPKD mice. Interference with inflammasome activation in the ADPKD animals resulted in better renal function and decreased IL18 excretion in the urine. These findings identify inflammasome inhibition as a promising concept for the treatment of ADPKD.

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Genome-wide analysis of renal gene expression during hyperoxaluria development

Project description:Previous studies have proposed that production of reactive oxygen species (ROS) is an important contributor to renal injury and inflammation following exposure to oxalate or calcium-oxalate crystals. The present study was conducted to determine, utilizing global transcriptome analyses, if the NADPH oxidase system is activated in kidneys of rats fed a diet leading to hyperoxaluria and crystal deposition. HLP was used to induce hyperoxaluria. Hyperoxaluria will lead to crystallization and up regulation of various NADPH oxidase subunits followed by increased expression of different specific genes. It is our hypothesis that crystallization induces inflammation of the kidneys via the activation of renin-angiotensin system (RAS) and production of reactive oxygen species (ROS) through NADPH oxidase complex. Apocynin was used to block hyperoxaluria and production of reactive oxygen species (ROS) with the inhibition of NADPH oxidase system as Apocynin inhibits membrane translocation of p47 subunit of NADPH oxidase. The present study was designed based on NADPH oxidase system and rats were divided into 4 groups (n= 6/group). Group 1 was fed regular rat chow diet, Group 2 received regular rat chow diet supplemented with 5% (Hydroxy-L-Proline) HLP, Group 3 received rat chow diet with 4 mmol Apocynin to drink, and Group 4 received regular rat chow diet with 5% HLP and 4 mmol Apocynin. After 28 days each rat was euthanized, their kidneys freshly explanted and dissected to obtain both cortex and medulla tissues. So the 4 groups were divided into cortex and medulla forming 8 groups. RNA was isolated from all the 8 specimens

2012-12-20 | E-GEOD-36446 | biostudies-arrayexpress

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