Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis in HEK293T WT and SCARNA15-KO cells

ABSTRACT: RNA-seq analysis in HEK293T WT and SCARNA15-KO cells

PROVIDER: PRJNA657623 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12463400_1.fastq.gz	Fastqsanger.gz
	SRR12463400_2.fastq.gz	Fastqsanger.gz
	SRR12463401_1.fastq.gz	Fastqsanger.gz
	SRR12463401_2.fastq.gz	Fastqsanger.gz
	SRR12463396_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

YTHDF1 CLIP-seq in WT and DHX36-KO HEK293T cells

Project description:To identify the YTHDF1 binding changes upon DHX36 loss, we conducted and analyzed YTHDF1 CLIP-seq data in WT and DHX36-KO HEK293T cells.

2024-10-03 | GSE264498 | GEO

YTHDF1 CLIP-seq in WT and DHX36-KO HEK293T cells

Project description:YTHDF1 CLIP-seq in WT and DHX36-KO HEK293T cells

| PRJNA1102848 | ENA

Characterization of HEK293T-SPINDOC KO vs WT by RNA-Seq profiling

Project description:Characterization of HEK293T-SPINDOC KO vs WT by RNA-Seq profiling

| PRJNA704262 | ENA

Anti-Pol II pS5 co-immunoprecipitation in PHF3 WT, KO and ΔSPOC HEK293T cells

Project description:The analysis of differential interactome of Pol II pS5 in PHF3 WT, KO and ΔSPOC HEK293T cells using Pol II pS5 (4H8) antibody coupled to Protein G beads.

2023-10-25 | PXD039537 | Pride

Anti-Pol II pS5 co-immunoprecipitation in DIDO WT, KO and ΔSPOC HEK293T cells

Project description:The analysis of differential interactome of Pol II pS5 in DIDO WT, KO and ΔSPOC HEK293T cells using Pol II pS5 (3E8) antibody coupled to Protein G beads (no crosslinking).

2023-10-25 | PXD039567 | Pride

Differential gene expression among HEK293T WT, LRPPRC-KO, LRPPRC-KO + WT, and LRPPRC-KO + Leigh Syndrome French Canadian type LRPPRC

Project description:Differential gene expression among HEK293T WT, LRPPRC-KO, LRPPRC-KO + WT, and LRPPRC-KO + Leigh Syndrome French Canadian type LRPPRC

| PRJNA915041 | ENA

Genome wide analysis of transcriptome modification after re-expression of PBRM1 WT and its BAH domain mutant in PBRM1 CRISPR-KO HEK293T cells.

Project description:Purpose: To find out if cancer-associated mutation in BAH domain affects gene expression? Method: We used PBRM1 CRISPR KO HEK293T cells and ectopically expressed GFP-PBRM1-WT, GFP-PBRM1 P1048R, GFP-PBRM1 T1202K and GFP-PBRM1 C1233W plasmid for 48 hours. Result: We found that GFP-PBRM1 WT and GFP-PBRM1 T1202K mutant were identical in terms of gene expression. However, mutant P1048R and C1233W had very little difference in expression of only 15 (14 up- and 1 down) out of 10,160 genes.

2021-09-20 | GSE149874 | GEO

Differential complexome profiling of CRISTA WT and KO HEK293T mitochondria

Project description:CRISTA (SMIM26) is a mitochondrial microprotein important for assembly of complex I. Here we used differential complexome profiling to study the composition of ETC complexes in SMIM26 WT and KO HEK293T mitochondria. CRISTA potentiates mitochondrial serine import via direct interactions with the serine transporter SFXN1 in the inner mitochondrial membrane. Its C-terminus furthermore interacts with the mitochondrial ribosome to form a SFXN1-CRISTA-mitoribosome triad that is enriched for mt-ND5 mRNA encoding a key enzymatic subunit of Complex I. Deletion of CRISTA reduces mitochondrial serine uptake, leading to reduced levels of folate intermediates. This in turn impairs 5-taurinomethyl(thio)uridinylation of mitochondrial tRNAs (tm5U and tm5S2U), leading to a specific loss of ND5 translation and a stall in Complex I biogenesis.

2025-06-18 | PXD047664 | Pride

Mitoribosome profiling (MitoRiboSeq) data from HEK293T wild-type, TACO1 knockout, and TACO1 reconstituted cells

Project description:Mitochondrial translation was investigated by mitochondrial ribosome profiling (mitoRiboSeq) in three HEK293T cell lines: HEK293T wild-type (WT), TACO1 knockout (TACO1-KO), and TACO1 reconstituted (TACO1-KO + TACO1) cells

2025-01-29 | E-MTAB-14071 | biostudies-arrayexpress

iNKT cells RNA-Seq (WT vs SFR KO)

Project description:iNKT cells RNA-Seq (WT vs SFR KO)

| PRJNA486367 | ENA