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ABSTRACT: GAI rRNA sequencing

PROVIDER: PRJNA661093 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR12576600_1.fastq.gz	Fastqsanger.gz
	SRR12576600_2.fastq.gz	Fastqsanger.gz
	SRR12576601_1.fastq.gz	Fastqsanger.gz
	SRR12576601_2.fastq.gz	Fastqsanger.gz
	SRR12576602_1.fastq.gz	Fastqsanger.gz

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Project description:Ovule development is a key process for plant reproduction that ensures correct seed production. Understanding the molecular mechanisms that control ovule formation will also provide new approaches to increase crop yield for breeding. Several molecular factors and plant hormones, including gibberellins, are involved in ovule initiation and development. Gibberellins control ovule development by the destabilization of DELLA proteins, whereas DELLA activity has been proved to act as a positive factor for ovule primordia emergence. But the molecular mechanism by which DELLA act remained unknown. Here we have proved that DELLA proteins control ovule initiation by the formation of a protein complex with the CUC2 transcription factor. The DELLA protein GAI requires CUC2 to promote ovule primordia formation, thus GAI would function by its direct protein-protein interaction with CUC2 in cells of the placenta that determine the boundary regions between ovules during pistil development. Analysis of GAI-CUC2 interaction and colocalization in placenta support this hypothesis. Moreover, molecular analysis of the loci at which GAI protein may act as transcriptional co-regulators in a CUC2-dependent manner identified a subset of target genes that would be regulated by the GAI-CUC2 complex and contribute to regulate ovule primordia emergence.

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