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Zeb1-knockdown retina endothelial cells

ABSTRACT: Zeb1-knockdown retina endothelial cells

PROVIDER: PRJNA668143 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12797022_1.fastq.gz	Fastqsanger.gz
	SRR12797022_2.fastq.gz	Fastqsanger.gz
	SRR12797023_1.fastq.gz	Fastqsanger.gz
	SRR12797023_2.fastq.gz	Fastqsanger.gz
	SRR12797024_1.fastq.gz	Fastqsanger.gz

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Transcriptome sequencing analysis of Zeb1-knockdown retina endothelial cells by RNA-seq

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Chromatin structure profiling of Zeb1-knockdown mouse retina endothelial cells by ATAC-seq

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Transcriptome sequencing analysis of Zeb1-knockdown retina endothelial cells by RNA-seq

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Chromatin structure profiling of Zeb1-knockdown mouse retina endothelial cells by ATAC-seq

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Zeb1-knockdown retina endothelial cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Expression data from Zeb1 knockdown NM18 cells

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Project description:Epithelial-to-mesenchymal transition (EMT) is a fundamental process in development and disease. If aberrantly activated it is a trigger for tumour progression and metastasis (Thiery et al 2009 Cell). It is now known that EMT activation is also associated with the maintenance of stem-cell properties (Mani et al. 2008 Cell). Since Zinc-finger enhancer binding transcription factor 1 (ZEB1) is a crucial EMT activator, we analyzed the changes in the gene expression profile that accompany shRNA mediated loss of ZEB1 in Panc1 pancreatic cancer cells. Panc1 is a cell line that exhibits relatively high ZEB1 levels and changes to a more benign phenotype upon ZEB1 knock down (Wellner et al. 2009 Nature Cell Biology). Panc1 cells were stably transfected with control (GFP) or ZEB1 shRNA. Upon puromycin selection, single cell clones were picked and characterized. Cells from two control versus two ZEB1 knockdown clones were harvested, total RNA was isolated and processed to hybridization.

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