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ABSTRACT: Nile tilapia temperature and infection challenge

PROVIDER: PRJNA670089 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12849337_1.fastq.gz	Fastqsanger.gz
	SRR12849337_2.fastq.gz	Fastqsanger.gz
	SRR12849338_1.fastq.gz	Fastqsanger.gz
	SRR12849338_2.fastq.gz	Fastqsanger.gz
	SRR12849339_1.fastq.gz	Fastqsanger.gz

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Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

Project description:In fish, the sex determining mechanisms can broadly be classified as genotypic (GSD), temperature-dependent (TSD), or genotypic plus temperature effects (GSD+TE). For the fish species with TSD or GSD+TE, extremely high or low temperature can affect its sex determination and differentiation. For long time, the underlying changes in DNA methylation that occur during high or low temperature induced sex reversal have not been fully clarified. In this study, we used Nile tilapia as a model to perform a genome-wide survey of differences in DNA methylation in female and male gonads between control and high temperature induced groups using methylated DNA immunoprecipitation (MeDIP). We identified the high temperature induction-related differentially methylated regions (DMRs), and performed functional enrichment analysis for genes exhibiting DMR. These identified differentially methylated genes were potentially involved in the connection between environmental temperature and sex reversal in Nile tilapia. In this study, four samples (control females, CF; control males, CM; induced females, IF; induced males, IM) were analyzed.

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Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

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Project description:We report an association of DNA hydroxymethylation profiling at single nucleotide resolution with gene expression in the fast muscle of Nile tilapia.

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Project description:Homeothermic vertebrates exposed to low temperature challenge activate multiple central and physiological pathways in order to regain homeostatic balance within the new environmental constrains. Such homeostatic responses include shivering and non-shivering thermogenesis, altered carbohydrate and lipid metabolism and protein catabolism synchronized by neuroendocrine responses. Pending their evolutionary life history, some of these physiological responses were also identified in poikilothermic vertebrates, yet a conception of heat seeking behavior and lack of cold-related metabolic regulation prevails. Using various physiological parameters and hypothalamic transcriptome analysis in the tropical poikilotherm Nile tilapia, we now demonstrate that cold stress in a poikilotherm induce complex central and physiological homeostatic responses. We further show that oxytocin which is a known thermoregulator in homeotherms, actively regulates temperature related homeostasis in poikilotherms. Moreover, we show that pharmacological or genetic blockage of oxytocin signaling affects oxygen-dependent metabolic rate in two cold exposed poikilothermic models. Therefore, our study identifies oxytocin as an adaptive and evolutionarily conserved metabolic regulator and show that poikilotherms brain actively respond to cold temperature stress by regulating metabolic physiology.

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Project description:Elongation of very long-chain fatty acids protein 6 (Elovl6) is a crucial enzyme in the synthesis of endogenous fatty acids, which participates in the energy balance and metabolic diseases. The main objective of this study was to explore the molecular characterization of elovl6 and study regulatory mechanism of elovl6 on male Nile tilapia, Oreochromis niloticus. In the present study, the full-length cDNA of Nile tilapia elovl6 was 2255 bp, comprising a 5'-UTR of 193 bp, an ORF of 810 bp, and a 3'-UTR of 1252 bp, encoding 269 amino acids that possesses the characteristic features of Elovl proteins. The transcription expression of elovl6 transcripts showed differential expression in different tissues and fed with different lipid sources diet. Antisense RNA technology was used to knock-down elovl6 in Nile tilapia. Compared with the control treatments (transfected with ultrapure water (control) or the blank expression vector (negative control)), knockdown elovl6 altered hepatic morphology, Long chain fatty acid synthesis, fatty acid oxidation, lead to excess fat deposition in the liver, ultimately cause abnormal glucose/lipid metabolism in male Nile tilapia. Transcriptome analyses (AGPAT2 knock-down vs. negative control) revealed a total of 5877 differentially expressed genes (DEGs). Transcriptome analyses revealed DEGs were greatly involved in some signaling pathways, such as PPAR signaling pathway, fatty acid degradation, glycolysis/gluconeogenesis, and insulin signaling pathway, which were related to lipid and glucose metabolism. We used qRT-PCR to verify the mRNA expression changes of 13 DEGs in related signaling pathways. Our results demonstrate that knock-down of elovl6 in tilapia blocks the synthesis of oleic acid and leads to abnormal glucose/lipid metabolism.

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