Project description:A marine bacterial isolate, Brevibacterium casei was grown in LB broth in presence of 50ppm Cadmium (provided in the form cadmium chloride, with final concentration of Cadmium at 50ppm). The cells were harvested at different time intervals and proteins extracted from these using indigenous protocol developed in laboratory. Similarly, the strain was grown in absence of Cadmium and same steps were followed, which was treated as control. The proteins extracted from test as well control set were digested using trypsin following standard protocol. Trypsin digests were injected in LCMS QToF for obtaining spectral library which was used to identify the proteins against NCBI database
