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Streptococcus pyogenes M1 GAS strain:SF370

ABSTRACT: Amplicon sequencing data from omega-dSpCas9 library selection pools

PROVIDER: PRJNA673046 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12958215_1.fastq.gz	Fastqsanger.gz
	SRR12958215_2.fastq.gz	Fastqsanger.gz
	SRR12958216_1.fastq.gz	Fastqsanger.gz
	SRR12958216_2.fastq.gz	Fastqsanger.gz
	SRR12958217_1.fastq.gz	Fastqsanger.gz

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Streptococcus pyogenes M1 GAS isolate:SF370

Project description:RNase III and Y SCPs in Streptococcus pyogenes

| PRJNA341675 | ENA

Streptococcus pyogenes M1 GAS isolate:SF370

Project description:Differential gene expression in Streptococcus pyogenes WT, Δrnc and Δrny

| PRJNA341673 | ENA

Streptococcus pyogenes M1 GAS strain:SF370

Project description:Streptococcus pyogenes M1 GAS strain:SF370 Genome sequencing

| PRJNA1114622 | ENA

Acquisition of the Sda1-encoding bacteriophage does not enhance virulence of the serotype M1 Streptococcus pyogenes strain SF370.

Project description:The resurgence of invasive disease caused by Streptococcus pyogenes (group A Streptococcus [GAS]) in the past 30 years has paralleled the emergence and global dissemination of the highly virulent M1T1 clone. The GAS M1T1 clone has diverged from the ancestral M1 serotype by horizontal acquisition of two unique bacteriophages, encoding the potent DNase Sda1/SdaD2 and the superantigen SpeA, respectively. The phage-encoded DNase promotes escape from neutrophil extracellular traps and is linked to enhanced virulence of the M1T1 clone. In this study, we successfully used in vitro lysogenic conversion to transfer the Sda1-encoding phage from the M1T1 clonal strain 5448 to the nonclonal M1 isolate SF370 and determined the impact of this horizontal gene transfer event on virulence. Although Sda1 was expressed in SF370 lysogens, no capacity of the phage-converted strain to survive human neutrophil killing, switch to a hyperinvasive covRS mutant form, or cause invasive lethal infection in a humanized plasminogen mouse model was observed. This work suggests that the hypervirulence of the M1T1 clone is due to the unique synergic effect of the M1T1 clone bacteriophage-specific virulence factor Sda1 acting in concert with the M1T1 clone-specific genetic scaffold.

| S-EPMC3676029 | biostudies-literature

Streptococcus pyogenes M1 GAS

Project description:Expression analysis of Streptococcus pyogenes isolate SF370 and its rgg2 mutant

| PRJNA246491 | ENA

Streptococcus pyogenes M1 GAS

Project description:Group A Streptococcus that causes a wide variety of infections

| PRJNA269 | ENA

Streptococcus pyogenes M1 GAS

Project description:Wild-type and irr mutant Group A Streptococcus (GAS) strains

| PRJNA89949 | ENA

Streptococcus pyogenes M1 GAS

Project description:Streptococcus pyogenes M1 GAS Transcriptome or Gene expression

| PRJNA516518 | ENA

Proteome driven re-evaluation and functional annotation of the Streptococcus pyogenes SF370 genome.

Project description:BackgroundThe genome data of Streptococcus pyogenes SF370 has been widely used by many researchers and provides a vast array of interesting findings. Nevertheless, approximately 40% of genes remain classified as hypothetical proteins, and several coding sequences (CDSs) have been unrecognized. In this study, we attempted a shotgun proteomic analysis with a six-frame database that was independent of genome annotation.ResultsNine proteins encoded by novel ORFs were found by shotgun proteomic analysis, and their specific mRNAs were verified by reverse transcriptional PCR (RT-PCR). We also provided functional annotations for hypothetical genes using proteomic analysis from three different culture conditions that were separated into three fractions: supernatant, soluble, and insoluble. Consequently, we identified 567 proteins on re-evaluation of the proteomic data using an in-house database comprising 1,697 annotated and nine non-annotated CDSs. We provided functional annotations for 126 hypothetical proteins (18.9% out of the 668 hypothetical proteins) based on their cellular fractions and expression profiles under different culture conditions.ConclusionsThe list of amino acid sequences that were annotated by genome analysis contains outdated information and unrecognized protein-coding sequences. We suggest that the six-frame database derived from actual DNA sequences be used for reliable proteomic analysis. In addition, the experimental evidence from functional proteomic analysis is useful for the re-evaluation of previously sequenced genomes.

| S-EPMC3224786 | biostudies-literature

Rgg-dependent transcriptional regulation in SF370 Streptococcus pyogenes strain

Project description:Rgg-dependent transcriptional regulation in SF370 Streptococcus pyogenes strain was analyzed during post-exponential phase of growth Keywords: rgg mutant

2008-02-01 | GSE7335 | GEO

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