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SLAMF5 blocking of sorted Bregs CD19+IL10+ (GFP+)

ABSTRACT: SLAMF5 blocking of sorted Bregs CD19+IL10+ (GFP+)

PROVIDER: PRJNA674605 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12979794.fastq.gz	Fastqsanger.gz
	SRR12979795.fastq.gz	Fastqsanger.gz
	SRR12979796.fastq.gz	Fastqsanger.gz
	SRR12979797.fastq.gz	Fastqsanger.gz

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SLAMF5 blocking of sorted Bregs CD19+IL10+ (GFP+)

Project description:Purpose: Our results show that in the absence of SLAMF5 ( by KO or blocking ab), there is an accumulation of Bregs (CD19+IL10+) and a specific increase of their survival. Here, using RNAseq, we wished to identify genes that take place in the regulation of Breg by SLAMF5 blocking. Method: Total RNA was extracted from 3x10e5 sorted CD19+GFP+cells from 2-3 Vert-x mice (induced with EAE) incubated with SLAMF5 blocking Ab or control IgG using the Direct-zol RNA kit (ZymoResearch) according to the manufacturer’s instructions. RNA-seq was performed using the MARS-seq protocol. Showing here 2 biological replicates of each group.

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