Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

N6-methyladenosine modification in chronic stress response due to social hierarchy positioning of mice

ABSTRACT: N6-methyladenosine modification in chronic stress response due to social hierarchy positioning of mice

PROVIDER: PRJNA675858 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13019200.fastq.gz	Fastqsanger.gz
	SRR13019201.fastq.gz	Fastqsanger.gz
	SRR13019202.fastq.gz	Fastqsanger.gz
	SRR13019203.fastq.gz	Fastqsanger.gz
	SRR13019204.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 22

Similar Datasets

N6-methyladenosine modification in chronic stress response due to social hierarchy positioning of mice

Project description:Appropriately responding to stressful events is important for maintaining health and well-being of an organism. With respect to social stress, the response is not always as straightforward as reacting to physical stressors, e.g. extreme heat, and thus has to be balanced subtly. Particularly, regulatory mechanisms contributing to gaining resilience in the face of mild social stress are not fully deciphered yet. We employed an intrinsic social hierarchy stress paradigm in mice of both sex to identify key factors for coping strategies. While global transcriptomic changes could not be observed in male mice, several genes which were previously reported to be involved in synaptic plasticity, learning and anxiety-like behavior were differentially regulated in female mice. Moreover, changes in N6-methyladenosine-modification of mRNA associated with corticosterone level occurred. Further investigation of proteins involved in m6A modification revealed that no significant changes of amount or activity could be detected for male mice. However, METTL3 and WTAP, subunits of the methyltransferase complex, showed elevated levels in female mice. N6-adenosyl-methylation is the most prominent type of mRNA methylation and plays a crucial role in processes such as metabolism, but also response to physical stress. Our findings underpin its important role by attributing it also to social stress evoked by hierarchy building within same-sex groups.

2020-11-11 | GSE161198 | GEO

social hierarchy

Project description:social hierarchy Raw sequence reads

| PRJNA1108947 | ENA

Distinct prefrontal projections oppositely orchestrate social competition and hierarchy

Project description:The medial prefrontal cortex (mPFC) is known as a hub of social hierarchy determination in the brain, Using high throughput single cell transcriptomic analysis and projection-specific genetic manipulation, we discovered that the expression level of Pou3f1 in mPFC-VTA neurons controls social hierarchy.

2023-12-11 | GSE222185 | GEO

N<sup>6</sup> -Methyladenosine Modification in Chronic Stress Response Due to Social Hierarchy Positioning of Mice.

Project description:Appropriately responding to stressful events is essential for maintaining health and well-being of any organism. Concerning social stress, the response is not always as straightforward as reacting to physical stressors, e.g., extreme heat, and thus has to be balanced subtly. Particularly, regulatory mechanisms contributing to gaining resilience in the face of mild social stress are not fully deciphered yet. We employed an intrinsic social hierarchy stress paradigm in mice of both sexes to identify critical factors for potential coping strategies. While global transcriptomic changes could not be observed in male mice, several genes previously reported to be involved in synaptic plasticity, learning, and anxiety-like behavior were differentially regulated in female mice. Moreover, changes in N6-methyladenosine (m6A)-modification of mRNA occurred associated with corticosterone level in both sexes with, e.g., increased global amount in submissive female mice. In accordance with this, METTL14 and WTAP, subunits of the methyltransferase complex, showed elevated levels in submissive female mice. N6-adenosyl-methylation is the most prominent type of mRNA methylation and plays a crucial role in processes such as metabolism, but also response to physical stress. Our findings underpin its essential role by also providing a link to social stress evoked by hierarchy building within same-sex groups. As recently, search for small molecule modifiers for the respective class of RNA modifying enzymes has started, this might even lead to new therapeutic approaches against stress disorders.

| S-EPMC8417747 | biostudies-literature

Social Hierarchy alters Drug Reward and the Protein Phosphorylation in the Nucleus Accumbens

Project description:Protein phosphorylation, a type of post-translational modification (PTM), has been identified in the addiction process. However, its role in social hierarchy remains poorly understood. In this study, we used the tube test to classify rats as dominants or subordinates. In the CPP paradigm, subordinates exhibited a higher susceptibility to addiction. Quantitative phosphoproteomic analysis revealed distinct phosphoproteomic profiles in the Nucleus Accumbens between these groups.

2025-04-09 | PXD062735 | Pride

Molecular and neural control of social hierarchy by forebrain-thalamocortical circuit

Project description:Many animal groups are organized hierarchically, which generates behavioral states that facilitate social interactions. Although generally stable, social status can change, underscoring the plasticity of underlying neural circuits. We examined competition among unfamiliar male mice and uncovered how the molecular and biophysical characteristics of a forebrain-thalamocortical circuit affect hierarchy. We identify the mediodorsal thalamus (MDT) as a hub receiving inputs from orbitofrontal cortex and basal forebrain and projecting to caudal anterior cingulate cortex (cACC) to regulate competitive performance. This circuit becomes potentiated or depressed in high- and low-rank males, respectively, in part through altered expression of the voltage-gated ion channel Trpm3 and synaptic plasticity. In high-rank mice, MDT projections drive inhibition of cACC pyramidal cells, promoting winning, in a pattern strikingly opposite to dorsomedial prefrontal cortex, where winners display increased pyramidal cell activity. Our data suggests a model in which hierarchy modulation relies on coordinated remodeling of multiple forebrain-thalamocortical circuits.

2025-11-13 | GSE300694 | GEO

Distinct prefrontal projections oppositely orchestrate social competition and hierarchy

Project description:Distinct prefrontal projections oppositely orchestrate social competition and hierarchy

| PRJNA918420 | ENA

Molecular and neural control of social hierarchy by forebrain-thalamocortical circuit

Project description:Molecular and neural control of social hierarchy by forebrain-thalamocortical circuit

| PRJNA1281677 | ENA

N6-methyladenosine Modulates Messenger RNA Translation Efficiency

Project description:N6-methyladenosine (m6A) is the most abundant internal messenger (mRNA) modification in mammalian mRNA. This modification is reversible and non-stoichiometric, which potentially adds an additional layer of variety and dynamic control of mRNA metabolism. The m6A-modified mRNA can be selectively recognized by the YTH family “reader” proteins. The preferential binding of m6A-containing mRNA by YTHDF2 is known to reduce the stability of the target transcripts; however, the exact effects of m6A on translation has yet to be elucidated. Here we show that another m6A reader protein, YTHDF1, promotes ribosome loading of its target transcripts. YTHDF1 forms a complex with translation initiation factors to elevate the translation efficiency of its bound mRNA. In a unified mechanism of translation control through m6A, the YTHDF2-mediated decay controls the lifetime of target transcripts; whereas, the YTHDF1-based translation promotion increases the translation efficiency to ensure effective protein production from relatively short-lived transcripts that are marked by m6A. PAR-CLIP and RIP was used to identify YTHDF1 binding sites followed by ribosome profling and RNA seq to assess the consequences of YTHDF1 siRNA knock-down

2015-06-09 | E-GEOD-63591 | biostudies-arrayexpress

N6-Methyladenosine methylation profile in rat H9C2 cardiomyocytes under the hypoxic stress

Project description:Hypoxia as a crucial pathogenesis factor usually results in huge harmful effects on cardiac injury and dysfunction. In our previous study (PMID: 33294289), We observe a series of differential expressed genes between transcription and translation, which may be attributed to the hypoxia-specific binding affinity of Nuclear cap-binding subunit 3 (NCBP3) at 5’ un-translation region of target genes. But the underlying molecular mechanism of NCBP3 for gene translation modulation remains unclear. Here, we conducted RIP-seq of N6-Methyladenosine methylation in H9C2 cells with the conditions of normoxic, hypoxic and with additional NCBP3 knockdown.

2021-06-30 | E-MTAB-10682 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data