Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin states and Transcriptome in in glioblastoma stem cells

ABSTRACT: Chromatin states and Transcriptome in in glioblastoma stem cells

PROVIDER: PRJNA678310 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13057268_1.fastq.gz	Fastqsanger.gz
	SRR13057268_2.fastq.gz	Fastqsanger.gz
	SRR13057269_1.fastq.gz	Fastqsanger.gz
	SRR13057269_2.fastq.gz	Fastqsanger.gz
	SRR13057270_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

Project description:Chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

| PRJNA818368 | ENA

Chromatin states in glioblastoma stem cell [ChIP-seq]

Project description:Chromatin states in glioblastoma stem cell [ChIP-seq]

| PRJNA678318 | ENA

RNA-seq of Glioblastoma stem cells

Project description:Chromatin accessibility discriminates stem from mature cell populations, enabling the identification of primitive stem-like cells in primary tumors, such as Glioblastoma (GBM) where self-renewing cells driving cancer progression and recurrence are prime targets for therapeutic intervention. We show, using single-cell chromatin accessibility, that primary GBMs harbor a heterogeneous self-renewing population whose diversity is captured in patient-derived glioblastoma stem cells (GSCs). In depth characterization of chromatin accessibility in GSCs identifies three GSC states: Reactive, Constructive, and Invasive, each governed by uniquely essential transcription factors and present within GBMs in varying proportions. Orthotopic xenografts reveal that GSC states associate with survival, and identify an invasive GSC signature predictive of low patient survival. Our chromatin-driven characterization of GSC states improves prognostic precision and identifies dependencies to guide combination therapies.

| EGAS00001003070 | EGA

ChromID reveals the proteome composition of key chromatin states in murine stem cells

Project description:Chromatin modifications instruct genome function through spatiotemporal recruitment of regulatory factors to the genome. However, how these modifications define the proteome composition at distinct chromatin states remains to be fully characterized. Here, we made use of natural protein domains as modular building blocks to develop engineered chromatin readers (eCRs) selective for histone and DNA modifications. By stably expressing eCRs in mouse embryonic stem cells and measuring their subnuclear localisation, genomicdistribution and histone-PTM-binding preference, we first demonstrate their applicability as selective chromatin binders in living cells. Finally, we exploit the binding specificity of eCRs to establish ChromID, a new method for chromatin-dependent proteome identification based on proximity biotinylation. We use ChromID to reveal the proteome at distinct chromatin states in mouse stem cells, and by using a synthetic dual-modification reader, we furthermore uncover the protein composition at bivalent promoters marked by H3K4me3 and H3K27me3. These results highlight the applicability of ChromID as novel method to obtaina detailed view of the protein interaction network determined by the chemical language on chromatin.

2020-04-30 | PXD014483 | Pride

Transcriptome of glioblastoma stem cells.

Project description:Transcriptome of glioblastoma stem cells.

| PRJNA678321 | ENA

Lactylome of 6 Glioblastoma Stem Cells and 2 Neural Stem Cells

Project description:Lactylome of 6 Glioblastoma Stem Cells and 2 Neural Stem Cells

2025-10-27 | PXD070007 |

Chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

Project description:Glioblastoma multiform account for about half of all gliomas and are the most deadly and aggressive forms. Its therapeutic resistance and tumor relapse rely on a subpopulation of cells, the so-called Glioma-stem Cells (GSCs). Here, we investigated for the role of the long non-coding RNA HOXA-AS2 in GSC biology by conducting descriptive and functional analyses of glioma samples classified according to their isocitrate dehydrogenase (IDH1) gene mutation status, and of glioma stem cells. We found that HOXA-AS2 is overexpressed only in aggressive (IDHwt) glioma and GSC. Sh-RNA-based depletion of HOXA-AS2 affects GSC both at the cellular and molecular levels with a decrease in proliferation and altered expression of several hundreds of their genes. Integrative analysis revealed that these changes is expression are not associated to changes in DNA methylation or chromatin signature at the promoter of most deregulated genes following HOXA-AS2 silencing in GSC, supporting a post-transcriptional regulation. In addition, transcription factor motif enrichment and correlation analyses sustained that HOXA-AS2 affect, directly or indirectly, expression of key transcription factors of GCS biology, including E2F8, E2F1, STAT1 and ATF3 to, in fine, contributes to their pathological status by promoting proliferation and modulating the inflammation pathway of Glioma Stem Cell.

2022-04-15 | GSE199031 | GEO

Proteome of 6 Glioblastoma Stem Cells and 2 Neural Stem Cells

Project description:Proteomic Analysis of 6 Glioblastoma Stem Cells and 2 Neural Stem Cells

2025-10-27 | PXD069969 |

Transcriptome and EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression), and chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

Project description:Transcriptome and EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression), and chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

| PRJNA818370 | ENA

Glioblastoma stem-like-cells

Project description:Glioblastoma stem-like-cells

| PRJNA751558 | ENA