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Gene expression profile in MS medium for the phytopathogenic fungus Fusarium graminearum

ABSTRACT: Gene expression profile in MS medium for the phytopathogenic fungus Fusarium graminearum

PROVIDER: PRJNA683251 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13215569_1.fastq.gz	Fastqsanger.gz
	SRR13215569_2.fastq.gz	Fastqsanger.gz
	SRR13215570_1.fastq.gz	Fastqsanger.gz
	SRR13215570_2.fastq.gz	Fastqsanger.gz
	SRR13215571_1.fastq.gz	Fastqsanger.gz

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Gene expression profile in MS medium for the phytopathogenic fungus Fusarium graminearum

Project description:Full transcriptome of the Fusarium graminearum wild-type strain CBS185.32, grown in MS medium, was studied.

2021-12-04 | GSE162839 | GEO

RNA-seq analysis of Fusarium graminearum Z-3639 in heat stress

Project description:Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. To dissect cellular responses toward heat stress in the plant pathogenic fungus F. graminearum, we compared transcriptomes of the fungal cultures incubated in normal temperature condition (25 ÂºC) and in high temperature condition (37 ÂºC) for 15 min. 6 samples examined: 24 h-old mycelia from complete medium (CM) of Fusarium graminearum wild-type Z-3639 were incubated in normal temperature condition (25 ÂºC) and in high temperature condition (37 ÂºC) for 15 min.

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Gene expression profile in MS medium for the phytopathogenic fungus Leptosphaeria maculans brassicae (Lmb) and lepidii (Lml)

Project description:Gene expression profile in MS medium for the phytopathogenic fungus Leptosphaeria maculans brassicae (Lmb) and lepidii (Lml)

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Fusarium graminearum in vitro secretome

Project description:The plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of the suite of proteases secreted. We used a systems biology approach comprising genome analysis, transcriptomics and label-free quantitative proteomics to characterise the peptidases deployed by Fgr during growth. A combined analysis of published microarray transcriptome datasets revealed seven transcriptional groupings of peptidases based on in vitro growth, in planta growth, and sporulation behaviours. An orbitrap MS/MS proteomics technique defined the extracellular proteases secreted by Fusarium graminearum.

2015-10-06 | PXD002786 | Pride

Fusarium graminearum in vitro cellular proteome

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RNA-seq analysis of Fusarium graminearum Z-3639 in heat stress

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Gene expression profiles in ML medium for the phytopathogenic fungus Botrytis cinerea

Project description:Gene expression profiles in ML medium for the phytopathogenic fungus Botrytis cinerea

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Comparative transcriptomics reveal contrasting fungal strategies in a plant pathogen (Fusarium graminearum) versus an endophyte (Metarhizium anisopliae) during initial host colonization

Project description:Conidial germination marks the beginning of the fungal life cycle, and understanding the genes associated with conidial germination provides insights into fungal pathogenicity and host interactions. Here, we use comparative transcriptomics to demonstrate the transcriptional similarities and differences during conidial germination and initial colony establishment in a plant pathogenic and an endophytic fungus, Fusarium graminearum and M. anisopliae, respectively. We compared the transcriptomes of F. graminearum and M. anisopliae across four stages of conidial germination: fresh conidia, polar growth, hyphal extension, and either first hyphal branching (on medium) or appressorium formation (on barley). F. graminearum exhibited a higher upregulation of CAZyme, specialized metabolite and effector genes compared to M. anisopliae during interaction with the host, particularly in the appressorium stage, reflecting its pathogenic nature. The appressorium structures formed when M. anisopliae conidia germinated on the host. The transcriptome analysis revealed that the fungus produced reduced transcript levels of CAZyme and specialized metabolite genes reflecting a less aggressive host penetration approach. The candidate genes associated with IAA synthesis were upregulated in M. anisopliae during the appressorium stage, supporting its endophytic lifestyle and suggests that the fungus uses a phytohormone based strategy to interact with plant hosts. Collectively, our findings expand the transcriptome resources and provide valuable insights into the gene networks involved in conidial germination and initiation of infection in pathogenic versus endophytic fungus.

2024-11-05 | GSE277787 | GEO

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Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes) and developing seeds. This study compare the gene expression profile in wheat spikelets (spk 2) inoculated with either water (mock treatment) or a pathogenic strain of Fusarium graminearum (WT); spikelets 2 were inoculated 24 hrs after a neighbour spikelet (spk 0) was treated with either water or F. graminerum mutant strain Tri6Δ or NoxABΔ. Spikelets 2 were sampled 8 and 24 hrs after the second treatment.

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Project description:Amycolatopsis sp. BX17 is an actinobacterium isolated from milpa soils that antagonizes the phytopathogenic fungus Fusarium graminearum. Metabolites secreted by the actinobacterium cultured in medium without glucose inhibited 100% the mycelial growth of F. graminearum RH1, while in medium supplemented with 20 g/L of glucose inhibition was 65%. With the aim of studying how the metabolism of strain BX17 is modulated by glucose, as the main carbon source, media with 0 and 20 g/L glucose were selected to analyze the intracellular proteins by quantitative label-free proteomic analysis.

2022-02-14 | PXD028644 | Pride

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