Project description:Hepatic complications of HCV infection, including fibrosis and cirrhosis are accelerated in HIV-infected individuals. Although liver biopsy remains the gold standard for staging HCV-associated liver disease, this test can result in serious complications and is subject to sampling error. These challenges have prompted a search for non-invasive methods for liver fibrosis staging. To this end, we compared serum proteome profiles at different stages of fibrosis in HIV/HCV co- and HCV mono-infected patients using SELDI-TOF MS.
Project description:TMT10plex global proteomics of HCV infected HuH7.5 cells or HCV infected humanized mouse livers. TMT channels were combined after labeling and separated off line with a highPH reverse phase fractionation into 24 fractions.
Project description:126 Peripheral whole blood samples from 26 individuals across two independent cohorts were collected prior to clozapine initiation, 4-12 weeks after initiation, and a 6 months after initiation.
Project description:HCV+ cells were detected by smiFISH on liver section of a patient with hepatocellular carcinoma. Clusters of HCV+ and HCV- cells were captured by laser microdissection and transriptomes analysed by RNA sequening. Highly sensitive single molecule fluorescent in situ hybridization applied to frozen tissue sections of a hepatitis C patient allowed the delineation of clusters of infected hepatocytes. Laser micro-dissection followed by RNAseq analysis of HCV-positive and -negative regions from the tumoral and non-tumoral tissues from the same patient revealed HCV-related deregulation of expression of genes in the tumor and in the non-tumoral tissue.
Project description:We performed miRNA array analysis for S-HCV (short-term HCV), L-HCV (long-term HCV) and control (uninfected Huh751), total for 3 samples to identify miRNA candidates in HCV-related HCC.