Robust Three-dimensional (3D) Expansion of Bovine Intestinal Stem Cells: A Model for Replacement of Animal Experiments Toward Animal Welfare
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA686090 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR13268228.fastq.gz | Fastqsanger.gz | |||
| SRR13268229.fastq.gz | Fastqsanger.gz | |||
| SRR13268230.fastq.gz | Fastqsanger.gz | |||
| SRR13268231.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 4 of 4 |
Similar Datasets
Project description:we successfully developed bovine intestinal organoids representing typical crypt-villus and epithelium’s characteristics from bovine small intestinal (jejunum) crypts
2021-07-28 | GSE163425 | GEO
Project description:Literary fiction has been credited with considerable power to improve attitudes toward outgroups. It was even argued that it has been an important factor behind the global decline of violence against various minorities in the last centuries. Could it also help to reduce the human-inflicted suffering of animals? To test this, we studied the attitude toward animal welfare of n = 921 (experimental group) people of both sexes who read a short fragment of an unpublished novel with a motif of the physical abuse of an animal. The control group (n = 912) read a fragment of a similar length but not related to animals. After reading the text all subjects filled out an on-line questionnaire with seven items (camouflaged among many others items) measuring attitudes toward animal welfare. The questionnaire included also demographical questions, such as whether the subject keeps pets. We found that in comparison with the control group, the experimental group was significantly more concerned about animal welfare. This result indicates that literary fiction can influence attitudes toward other species. It is also worth noting that our study is characterized by a high level of ecological validity, i.e. a relatively high extent to which its results can be generalized (or extended) to real-world settings. Due to its specific design, which involved the cooperation of a bestselling author and his publisher, the study approximated the typical conditions in which people read fiction in a remarkably accurate way. Finally, our research has potential practical implications for promoting animal welfare.
| S-EPMC5179074 | biostudies-literature
Project description:[This corrects the article DOI: 10.1371/journal.pone.0168695.].
| S-EPMC5302374 | biostudies-literature
Project description:A comprehensive understanding of current Chinese public attitudes toward farm animal welfare and the relevant influencing factors is essential for improving farm animal welfare and promoting further development of animal husbandry. The attitudes of 3,726 respondents in China were investigated using paper and online questionnaires. Three components (affective, cognitive, and behavioral) of attitudes toward farm animal welfare were assessed using 18 items designed based on the literature review. Influential factors of attitudes toward farm animal welfare were explored via tobit regression. The results revealed that the Chinese public not only considers farm animals to be emotional and sentient but are also sympathetic toward farm animals that suffer inhumane treatment. Although they have limited knowledge about farm animal welfare, the public believes improving farm animal welfare is beneficial, especially for food safety and human health. The Chinese public prefers regulation policies to incentive policies for improving farm animal welfare. The main factors influencing attitudes toward farm animal welfare included gender, age, education, monthly household income, area of residence, farm animal raising experience, and attention to farm animal welfare events. The effect of these influencing factors on attitudes varied. These findings provide a basis for improving Chinese public attitudes toward farm animal welfare. The implications of formulating and implementing effective policies to improve the Chinese public attitudes toward farm animal welfare were discussed.
| S-EPMC10035598 | biostudies-literature
Project description:Beef represents a major diet component and source of protein in many countries. With an increment demand for beef, the industry is currently undergoing changes towards natural produced beef. Consumers not only concern about product quality, but also for the well-being of animals. Therefore, the consumption of grass-fed meat is continuously growing. However, the nutritional true differences between feeding systems are still unclear. The aim of this study was to examine latissimus dorsi muscle quality and animal welfare by transcriptome and metabolome profiles, and to identify biological pathways related to the differences between grass- and grain-fed Angus steers. By RNA-Seq analysis of latissimus dorsi muscle, we have recognized 241 differentially expressed genes (FDR < 0.1). The metabolome examination of muscle and blood revealed 163 and 179 altered compounds in each tissue (P-value < 0.05), respectively. Accordingly, alterations in glucose metabolism, divergences in free fatty acids and carnitine conjugated lipid levels, and altered β-oxidation, have been observed. In summary, this study demonstrates a unique transcriptomic and metabolic signature in the muscle of grain and grass finished cattle. Results support the accumulation of anti-inflammatory n3 polyunsaturated fatty acids in grass finished cattle, while higher levels of n6 PUFAs in grain finished animals may promote inflammation and oxidative stress. Furthermore, grass-fed animals produce tender beef with lower total fat and higher omega3/omega6 ratio than grain fed animals, which could potentially benefit consumer health. Finally, blood cortisol levels strongly indicate that grass fed animals experience less stress than the grass fed individuals
2016-06-23 | GSE70248 | GEO
Project description:Beef represents a major diet component and source of protein in many countries. With an increment demand for beef, the industry is currently undergoing changes towards natural produced beef. Consumers not only concern about product quality, but also for the well-being of animals. Therefore, the consumption of grass-fed meat is continuously growing. However, the nutritional true differences between feeding systems are still unclear. The aim of this study was to examine latissimus dorsi muscle quality and animal welfare by transcriptome and metabolome profiles, and to identify biological pathways related to the differences between grass- and grain-fed Angus steers. By RNA-Seq analysis of latissimus dorsi muscle, we have recognized 241 differentially expressed genes (FDR < 0.1). The metabolome examination of muscle and blood revealed 163 and 179 altered compounds in each tissue (P-value < 0.05), respectively. Accordingly, alterations in glucose metabolism, divergences in free fatty acids and carnitine conjugated lipid levels, and altered β-oxidation, have been observed. In summary, this study demonstrates a unique transcriptomic and metabolic signature in the muscle of grain and grass finished cattle. Results support the accumulation of anti-inflammatory n3 polyunsaturated fatty acids in grass finished cattle, while higher levels of n6 PUFAs in grain finished animals may promote inflammation and oxidative stress. Furthermore, grass-fed animals produce tender beef with lower total fat and higher omega3/omega6 ratio than grain fed animals, which could potentially benefit consumer health. Finally, blood cortisol levels strongly indicate that grass fed animals experience less stress than the grass fed individuals The steers came from a closed Wye Angus herd with very similar genetics. The grass-fed group was comprised of steers that received alfalfa and orchard grass hay, clover and orchard grass pasture, or orchard grass and alfalfa pasture. The grass-fed individuals consumed grazed alfalfa upon availability and bales during winter and were not exposed to any corn, any form of grain or feed by-products. The alfalfa and grass hay were harvested from land that has had minimal fertilizer and no application of pesticides or inorganic chemicals. The control group was fed a conventional diet consisting of corn silage, soybean, shelled corn and minerals. The pastures were managed as organic landsâwithout fertilizers, pesticides or any chemical additives. At the slaughter plant, 10 ml whole blood sample from the jugular vein was collected in EDTA tubes and directly storage at -80°C. Then, a small piece of longissimus dorsi muscle was obtained from each hot carcass at the level of the 12th intercostal space and immediately frozen in dry ice for posterior analysis.
2016-06-23 | E-GEOD-70248 | biostudies-arrayexpress
Project description:Sporulation: TAO3 replacement strains
| PRJEB8698 | ENA
Project description:This study compared the brain proteome response of fish submitted to cold shock by fast-chilling (FC) as a novel stunning method (with fish immersed in liquid water at -8ºC), asphyxia (ASP) (as the currently used method), electrical stunning (ES) and anaesthesia (AN) with MS-222, by integrating it with physiological, behavioural and quality welfare indicators. Fish trials were conducted in triplicate tanks and two fish per tank were randomly sampled for MS analysis. One of the objectives of this integrative work was to disclose most significant changes in the brain proteome of rainbow trout (O. mykiss) subjected to four types of stunning methods that could help to elucidate about welfare standards.
2024-01-10 | PXD044301 | Pride
Project description:Opinions increasingly influence legislative processes. The case of animal welfare (AW) standards is a clear example of the role played by opinions in political decisions. The issue, to which European citizens have historically been sensitive, has been the subject of numerous consultations and investigations, aimed at considering citizens' opinions regarding breeding conditions. However, these tools and in particular standard surveys, suffer from response biases such as the prejudices involved in the design of questions and the interpretation of the results. To mitigate these problems, we used the Q-methodology, which is an inductive but systematic methodology focused on patterns that explain the ideas of individuals. The purposive sample consisted of 36 veterinary students who were acquiring scientific knowledge about AW. The results, in addition to providing policymakers with food for thought for the further development of AW standards, contribute to testing the use of alternative methodologies to collect citizens' views. This mapping of viewpoints helps to build a more effective form of AW policy making.
| S-EPMC7460027 | biostudies-literature