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γδT cell Interactions within the Tissue Ecosystem Network [bulk RNAseq]

ABSTRACT: γδT cell Interactions within the Tissue Ecosystem Network [bulk RNAseq]

PROVIDER: PRJNA688581 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13326566.fastq.gz	Fastqsanger.gz
	SRR13326567.fastq.gz	Fastqsanger.gz
	SRR13326568.fastq.gz	Fastqsanger.gz
	SRR13326569.fastq.gz	Fastqsanger.gz
	SRR13326570.fastq.gz	Fastqsanger.gz

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γδT cell Interactions within the Tissue Ecosystem Network [bulk RNAseq]

Project description:Numerous lymphocytes seed the body surfaces of metazoans promoting tissue protection and integrity. These tissue-resident populations differ substantially from circulating lymphocytes, as they adopt a unique phenotype and do not recirculate. They span the innate-adaptive continuum, ranging from innate lymphoid cells (ILCs) to unconventional T cells (NKT, MAIT, γδT cells and IELs) and tissue-resident memory (TRM) T cells, and while differ in the particulars of their biology, all contribute to barrier immunity, tissue homeostasis, and immune regulation. Although they share the same microenvironment, little is known about how these tissue-resident lymphocyte populations interact with each other and with the non-lymphoid cellular environment. As such, there is an emerging view of tissues as varied ‘‘ ecosystems ’’, whereby protection against inflammatory challenges is orchestrated through the interplay between tissue-resident and infiltrating-lymphocyte subtypes. In mice, barrier tissues are populated during early life by innate-like γδT lymphocytes. These cells provide tissue protection, including limiting conventional T cell-driven inflammation. Therefore, elucidating the contributions of tissue-resident T cells, particularly γδT cells, to the basic pathophysiology of tissue function and protection against infection, inflammation, and cancer is a priority. To this aim, we have induced allergic contact dermatitis in the back skin of different strains and we analyzed the behavior of γδT cells

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Tissue-resident memory T cells (TRM) Interactions within the Tissue Ecosystem Network [scRNA-seq]

Project description:We aim to understand here, the influence of TCR-Vγ5+ γδT cells in the recruitment of TRM population. Along these lines we will analyse the populations of TRM recruited to the Skin after the challenge process (induction of allergic contact dermatitis), in the presence of TCR-Vγ5+ or TCR-Vγ5− (replacement population).

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Tissue-resident memory T cells (TRM) Interactions within the Tissue Ecosystem Network

Project description:Tissue-resident memory T cells (TRM) Interactions within the Tissue Ecosystem Network

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Project description:Tissue-resident memory T cells (TRM) Interactions within the Tissue Ecosystem Network [scRNA-seq]

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Single-cell Transcriptional Analysis Reveals Allergen-specific Signatures in Human γδT Cells

Project description:The role of gamma-delta T (γδT) cells in immune responses to common allergens is poorly understood. Here, we utilized single-cell (sc) transcriptomic analysis of allergen-reactive γδT cells in humans to characterize the transcriptional landscapes and TCR repertoires in response to cockroach (CR) and mouse (MO) allergens. Using a novel Activation-Induced Marker (AIM) assay that allows detection of γδT cells combined with scRNA sequencing and TCR repertoire analysis, we identified both shared and allergen-specific γδT cell activation patterns and gene expression profiles. While CR extract activated both Vδ1 and Vδ2 subsets, MO extract primarily stimulated Vδ2 cells. Our analysis revealed allergen-specific clusters with distinct functional signatures, including enhanced inflammatory responses and cytotoxic effector functions in MO-specific γδT cells and natural killer cell-mediated immunity and IFNγ signaling in CR-specific populations. Comparison of allergic and non-allergic individuals highlighted differences in gene expression and TCR repertoires, including a higher IFNG expression in the CR-allergic compared to non-allergic cohorts, suggesting that phenotypic and functional differences are associated with γδT allergen responses. This study provides insights into the cellular and molecular heterogeneity and functionality of allergen-reactive γδT cells, offering a foundation for understanding their role in allergic diseases and potential therapeutic interventions.

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Tissue-resident memory T cells (TRM) Interactions within the Tissue Ecosystem Network

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