Ontology highlight
ABSTRACT:
PROVIDER: PRJNA689632 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR13358757_1.fastq.gz | Fastqsanger.gz | |||
| SRR13358757_2.fastq.gz | Fastqsanger.gz | |||
| SRR13358758_1.fastq.gz | Fastqsanger.gz | |||
| SRR13358758_2.fastq.gz | Fastqsanger.gz | |||
| SRR13358759_1.fastq.gz | Fastqsanger.gz |
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Project description:Unsaturated fatty acids (UFAs) in beef are essential for human health. Long-chain fatty acyl-CoA synthase 1 (ACSL1) is a crucial gene for UFAs synthesis in bovine adipocytes. To assess the protein expression profile during UFAs synthesis, we performed a proteomic analysis of bovine adipocytes by RNA interference and non-interference with ACSL1 using label-free techniques.
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Project description:Systematic analyses of the temporal dynamics of transcriptomes and chromatin landscapes of macrophages during timecourse of TLR4-mediated inflammatory response. As a multifunctional effector cell, macrophages play pivotal roles in both the induction and resolution components of varied inflammatory processes. During the course of an inflammation response, macrophages engage in a homeostatic program characterized by tightly coordinated modulation of temporal outputs of both lipid metabolism and inflammation. We demonstrate inversely biphasic temporal dynamics of specific fatty acid metabolic and inflammatory gene expression profiles, associated with concordant temporal reprogramming of macrophage fatty acid profiles. In part, the late phase of the macrophage inflammatory response is characterized by tailoring of fatty acid related gene expressions, facilitating both significant induction of anti-inflammatory unsaturated fatty acid production and associated resolution of inflammation. We demonstrate the biphasic temporal dynamics of macrophage inflammation, specifically anti-inflammatory omega-3 and omega-9 unsaturated fatty acid levels, are transcriptionally driven genome-wide by an unexpected shift from an LXR to SREBP1-dominant regulatory program in the late phase inflammatory response. Collectively, our findings reveal a novel Srebp1-driven mechanism allowing the intimate inverse temporal relationship between the transcriptional regulation of inflammatory and fatty acid metabolic outputs; whereby modulation key transcriptional regulators (LXR, SREBP1 and NF-kB) of these pathways coordinate appropriate temporal tailoring of local enhancer associated reprogramming and eventual pathway regulatory interactions, during the course of TLR4-dependent inflammatory response in macrophages. This specific Srebp-driven, temporal reprogramming of macrophage fatty acid metabolism, characterized by late phase induction of anti-inflammatory unsaturated fatty acid production, is necessary for appropriate resolution of inflammation. Thus, this study suggests that selective reprogramming of macrophage lipid metabolism can serve as a viable therapeutic intervention aimed at ameliorating chronic inflammation and varied metabolic syndrome associated states.
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Project description:We applied single-cell ATAC sequencing and lipid profiling to inguinal and epididymal adipose depots from mice that received sham surgery or vertical sleeve gastrectomy (VSG). We observed depot-specific cellular composition and chromatin accessibility patterns that were altered by VSG. Specifically, accessibility at Scd1, a fatty acid desaturase, was substantially reduced after VSG in mature adipocytes of inguinal but not epididymal depots. This was accompanied by reduced accumulation of SCD1-produced unsaturated fatty acids. Given these findings and reports that reductions in Scd1 attenuate obesity and insulin resistance our results suggest VSG exerts its beneficial effects through an inguinal depot-specific reduction of SCD1 activity.
2021-12-20 | GSE191064 | GEO
Project description:The goal of this study was to compare the transcriptional responses of mouse macrophages treated with unsaturated or saturated fatty acids to macrophages treated with LPS to stimulate classical inflammatory activation. Microarray profiling was performed on total RNA isolated from primary mouse bone marrow-derived macrophages (BMDMs) treated with fatty acids (C18:1 oleic acid or C18:0 stearic acid), BSA vehicle, or LPS at two time points.
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Project description:The 21st century is an era of molecular breeding, leading to fundamental dairy market and production system changes. Milk fat is the main economic trait in dairy cattle production. Our previous study revealed that the ACADSB gene has a crucial regulatory role in lipid metabolism, which can alter the contents of TGs, CHOL and fatty acids by regulating some related gene which involve in lipid metabolism. As usual, when we explored the function of interest genes, we often design interference and overexpression vectors to regulate the expression level of related gene, and transiently transfect vectors into cells, however, it is limited by transfection efficiency. therefore, we committed to finding a stable mode of gene expression. Knocking out of the ACADSB gene in bovine mammalian epithelial cells (bMECs), which represents a promising and effective means of further research on the effects of ACADSB in vivo lipid metabolism of dairy cattle. At the same time, the knock-out ACADSB gene's bMECs could also be useful genetic material and tools for future research into gene functions related to lipid and fatty acid metabolism, which also can directly use for further exploring the gene molecular mechanism or related pathway.
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2020-01-01 | GSE137993 | GEO
Project description:Liver sinusoidal endothelial cells (LSEC) are unique endothelial cell typelining the sinusoids of the liver and we have shown that these cells respond in a unique matter when exposed to saturated and unsaturated free fatty acids (FFA) and bile acids. We used microarray to analyze the transcriptional differences between the LSEC exposed to free fatty acids and bile acid receptor agonists to further shed light on their role in non-alcoholic fatty liver disease. The Murine Liver Sinusoidal Endothelial Cell Line (TSEC) was treated with palmitic and oleic acid or the bile acid receptor agonist INT-767 for 8 hours. Total RNA was then harvested to determine transcriptional differences.
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