Project description:To assess the impact of surface water across the Hun River, several sampling sites located in the mainstream and the tributary were selected representative of pollution gradient and different pollution source. Male adult zebrafish were exposed to surface water from seven sites for 4 days. The obiectives of the study was to evaluate the ability of transcriptomic profiles exposed to surface water to determine the potential biological effects, to differentiate different pollution source, and to identify the toxic components.
Project description:To assess the impact of surface water across the Hun River, several sampling sites located in the mainstream and the tributary were selected representative of pollution gradient and different pollution source. Male adult zebrafish were exposed to surface water from seven sites for 4 days. The obiectives of the study was to evaluate the ability of transcriptomic profiles exposed to surface water to determine the potential biological effects, to differentiate different pollution source, and to identify the toxic components.
Project description:To assess the impact of surface water across the Hun River, several sampling sites located in the mainstream and the tributary were selected representative of pollution gradient and different pollution source. Human mesenchymal stem cells were exposed to organic extracts of surface water from six sites for 2 days. Microarrays were used to measure the gene expression. And the gene expression profiles were used to evaluate the ability of determine the potential biological effects, to differentiate different pollution source, and to identify the toxic components.
2018-06-01 | GSE110310 | GEO
Project description:Microorganism in water source reservoir
Project description:<p>Microplastics, antibiotics, and heavy metals are co-occurring pollutants in wastewater, posing significant environmental risks and potential threats to human health. However, there is currently no effective wastewater treatment method for this type of combined pollution. This study establishes a novel dual-chamber fungal system by leveraging fungi’s remarkable capacity to degrade multiple pollutants in complex environments and their responsiveness to micro-voltage stimulation. Following treatment with this system, up to 84.28±2.85% of enrofloxacin (ENR) and 95.52±1.77% of Pb were removed. Meanwhile, under the trapping effect of fungal mycelium, more than 95% of polypropylene, polystyrene and polyvinyl chloride microplastics distributed in different water layer areas were removed. Multi-omics analysis revealed that micro-voltage enhanced fungal energy, amino acid, and cofactor metabolism, thereby altering fungal surface structural properties and increasing extracellular oxidase activity. These alterations improved the entrapment and aging of microplastics by fungal, while simultaneously promoting the decomposition of ENR and the mineralization/adsorption of Pb on the fungal surface. Furthermore, experiments conducted in genuine wastewater confirmed the stability and security of the micro-voltage driven fungal system under practical operating conditions, including organic load and ammonia nitrogen shocks. This study offers novel insights and promising avenues for addressing combined pollution.</p>
Project description:Oil spills have polluted the marine environment for decades and continue to be a major source of polycyclic aromatic hydrocarbons (PAHs) to marine ecosystems around the globe. Although the toxicity of PAHs to fish has been well studied, the combined effects of extreme abiotic factors and oil are poorly understood. Gulf of Mexico killifish Fundulus grandis larvae (< 24 hours post hatch) were exposed to varying environmental conditions (dissolved oxygen 2, 6 ppm; temperature 20, 25, 30°C; and salinity 3, 10, 30 ppt) combined with varying concentrations of high energy water accommodated fractions (HEWAF) (total PAHs 0 – ~ 125 ppb) for a total of 48 h. Larvae survival and development were negatively affected by PAHs, starting with the lowest concentration tested (~15 ppb). High temperature + hypoxia + PAHs resulted in the lowest survival with salinity having little impact on any of the endpoints tested. Expression of the hepatic detoxifying gene cyp1a was highly induced in PAH-exposed larvae, but only under normoxic conditions. A lack of cyp1a induction under hypoxia and PAH exposure could explain the enhanced toxicity observed. This work highlights the need for more studies examining the combined impact of suboptimal water quality parameters in the presence of pollution in fish early life-stages.
2019-04-18 | GSE129998 | GEO
Project description:Faecal Pollution Source Tracking in the Ouseburn
Project description:We reported the gene expression profile of T47D cells treated with the organic extract of Particulate matter 2.5 (PM2.5) sampled next to the municipal solid waste incineration plant of Bologna city. Based on a air pollution distribution model that takes the incinaration plant as point source of emission, two sites were chosen to sample particulate matter near incineration plant: "FrulloEst" representing the maximum effect of the incineration plant, "Calamosco" representing the negative control of "FrulloEst" (minimun effect of incineration plant, same effect of other air pollution fonts). Another site, "Giardini Margherita", is chosen to sample the urban background air pollution. for each site sample collection was performed in winter and in summer season.