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Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease [scRNA-seq]

ABSTRACT: Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease [scRNA-seq]

PROVIDER: PRJNA691041 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13397562_1.fastq.gz	Fastqsanger.gz
	SRR13397562_2.fastq.gz	Fastqsanger.gz
	SRR13397563_1.fastq.gz	Fastqsanger.gz
	SRR13397563_2.fastq.gz	Fastqsanger.gz
	SRR13397564_1.fastq.gz	Fastqsanger.gz

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Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease.

Project description:Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease.

| PRJNA691039 | ENA

Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease [bulk RNA-seq]

Project description:Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease [bulk RNA-seq]

| PRJNA691042 | ENA

Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease [scRNA-seq]

Project description:To address the need for human alveolar epithelial cell (AEC)-derived lines to more suitably model distal lung diseases, we have generated and characterized novel immortalized cell lines derived from human AECs. We used a combination of the ROCK inhibitor, Y-27632, and lentiviral transduction of SV40 Large T antigen of previously cryopreserved isolated human alveolar epithelial type 2 (AT2) cells to generate immortalized AECs. These AEC lines proliferate well on standard tissue culture dishes forming an epithelial monolayer and express lung progenitor markers SOX9 and SOX2. When grown in 3D culture with lung fibroblasts, the cells form NKX2-1+ organoids expressing more mature alveolar lung markers, AQP5 and GPRC5A. Single cell RNA-sequencing of one AEC line comparing cells in 2D versus 3D revealed increased cellular heterogeneity and an induction of cytokine and lipoprotein signaling in 3D culture, reflecting interactions with the microenvironment during organoid formation. Taken together, these data show our novel progenitor-like AEC lines retain a genetic and structural memory of their alveolar cell lineage despite long-term expansion, providing a valuable new system to model the distal lung in vitro.

2022-01-10 | GSE164514 | GEO

Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease [bulk RNA-seq]

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Mouse Models of Alveolar/Embryonal Rhabdomyosarcoma & Spindle Cell Sarcomas

Project description:A series of conditional mouse models of embryonal rhabdomyosarcoma, alveolar rhabdomyosarcoma and spindle cell sarcoma were generated and validated for relavence to corresponding human cancers. Conditional mouse models of embryonal rhabdomyosarcoma, alveolar rhabdomyosarcoma and spindle cell sarcoma were created by activation or deletion of Pax3:Fkhr, p53, Ptch1 or Rb1 genes.

2011-02-17 | E-GEOD-22520 | biostudies-arrayexpress

Development of human alveolar epithelial cell models to study distal lung biology and disease.

Project description: Not available

| S-EPMC8829779 | biostudies-literature

Single cell transcriptome analysis of distal adult human lung airway cells

Project description:To comprehensively study the heterogeneity within distal airway epithelium, we performed single cell transcriptomic analysis of the normal human donor lung samples. Our analysis reveals that secretory (club) and basal cells in the distal lung airway epithelial cells are highly heterogenous. Further interrogation of secretory cells identified a subpopulation with potential for alveolar differentiation in vitro, implicating distal lung airway secretory cells as new candidates in alveolar repair and regeneration.

2021-12-10 | GSE130076 | GEO

Development of novel in vitro human alveolar epithelial cell models to study distal lung biology and disease.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Proteomics Analysis of Extracellular Vesicles from In Vitro Models of the Human Airway.

Project description:Extracellular vesicle (EV)-mediated intercellular communication significantly influences pulmonary cell health and disease outcomes, yet in vitro methods to investigate these pathophysiological mechanisms are limited. We hypothesize that organotypic models of the airway can be leveraged to investigate EV-mediated intercellular signaling, focusing on EV proteomic content as a case study. in this study, two in vitro airway culture models were evaluated using mass spectrometry-based proteomics: a tri-culture model consisting of alveolar epithelial, fibroblast, and lung microvascular endothelial cells and a co-culture model consisting of alveolar epithelial and fibroblast cells. EVs isolated from the tri-culture model showed enrichment for EV proteins regulating RNA-to-protein translation. EVs isolated from the co-culture model were enriched with EV biogenesis and extracellular matrix signaling proteins. These model-specific differences suggest that different pulmonary cell types uniquely affect EV composition and the biological pathways influenced by the EV proteome in recipient cells. These findings inform future study designs surrounding EV-related pulmonary disease pathogenesis and therapeutics.

2024-01-26 | PXD040470 | Pride

Distal airway stem cells yield alveoli in vitro and during lung regeneration following H1N1 influenza infection (colony)

Project description:The extent of lung regeneration following catastrophic damage and the potential role of adult stem cells in such a process remains obscure. Sublethal infection of mice with an H1N1 influenza virus related to that of the 1918 pandemic triggers massive airway damage followed by apparent regeneration. We show here that p63-expressing stem cells in the bronchiolar epithelium undergo rapid proliferation after infection and radiate to interbronchiolar regions of alveolar ablation. Once there, these cells assemble into discrete, Krt5+ pods and initiate expression of markers typical of alveoli. Gene expression profiles of these pods suggest that they are intermediates in the reconstitution of the alveolar-capillary network eradicated by viral infection. The dynamics of this p63-expressing stem cell in lung regeneration mirrors our parallel findings that defined pedigrees of human distal airway stem cells assemble alveoli-like structures in vitro and suggests new therapeutic avenues to acute and chronic airway disease. 3 colonies fron control and H1N1 influenza treated lungs (12 dpi) ,each were picked for whole genome microarray analysis

2011-11-01 | E-GEOD-32600 | biostudies-arrayexpress

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