Project description:Synapse formation and elimination are two crucial processes that concurrently take place in the developing brain. Astrocytes and microglia have been shown to control both processes. However, it is largely unknown how these two major glial cell types of the central nervous system (CNS) communicate to balance synapse formation and elimination. Astrocytes secrete a synaptogenic protein called Hevin/SPARCL1, which induces the formation and plasticity of thalamocortical synapses in the mouse visual cortex. Hevin does so by physically localizing to synaptic clefts and bridging the thalamic axon/cortical dendrite via its interactions with presynaptic Neurexin1a and postsynaptic Neuroligin1b. Here, we found that in addition to this synaptogenic function, Hevin directly signals to microglia cells by interacting with Toll-like Receptors (TLRs) TLR4 and TLR2. This signaling occurs when Hevin is proteolytically cleaved producing an active C-terminal fragment. This fragment is sufficient to upregulate TLR2 expression in microglia and increase microglia phagocytic activity in vivo. This signaling is required for proper refinement of thalamocortical synapses in early postnatal development and for early life ocular dominance plasticity.
Project description:Microglia were FACS-isolated from developing mouse corpus callosum at postnatal days 0, 7, and 21, then sequenced by 10X Genomics single-cell sequencing.
Project description:In this study, we established a high-throughput workflow for unbiased, simultaneous profiling of the single-cell proteome and cellular morphology, achieving high sensitivity and reproducibility. Using this workflow, we analyzed male mouse microglia sorted from hippocampus and prefrontal cortex at three distinct ages including 2, 14, and 24 months, generating a high-quality proteomic dataset (3,184 single cells after quality control; average of 1,339 protein groups per cell) with robust consistency.
Project description:This study defines a molecular interaction between neurons and microglia that drives experience-dependent synapse remodeling in the hippocampus.