Project description:To identify full-length circRNAs in lung normal and cancer cell lines, we enriched circRNAs by rRNA depletion, poly(A) tailing and Rnase R treatment and amplified circRNAs by rolling circular RT. We constructed library according to the protocol of Fangqing Zhao team's. Nanopore is used for full-length sequencing.
2025-05-27 | GSE262357 | GEO
Project description:Custom-primed rolling circle amplicons for highly accurate nanopore
| PRJNA1157368 | ENA
Project description:Rolling Circle Reverse Transcription Enables High Fidelity Nanopore Sequencing of Small RNA
Project description:We report isoCirc, a long-read sequencing strategy coupled with an integrated computational pipeline to characterize full-length circular RNA (circRNA isoforms) using rolling circle amplification (RCA) followed by long-read sequencing. Applying isoCirc to 12 human tissues, we determined full-length structures and examined tissue specificities of circRNA isoforms in human transcriptomes.
2020-09-12 | GSE141693 | GEO
Project description:Rolling circle of Yeast RNAP mutants
| PRJNA923308 | ENA
Project description:Paramecium tetraurelia rolling-circle at different temperatures
| PRJNA839092 | ENA
Project description:Rolling-circle of C. elegans RNAPII mutants
Project description:This study investigated changes in the transcriptome of outdoor grown leafy spurge crown buds as they progress from paradormancy in August and September into endo dormancy in October through to ecodormancy in November and December. Keywords: Dormancy leafy spurge adventitious-buds A series of balanced dyeswap rolling circle hybiridizations were used with each year representing s seperate circle and with direction of the circles reversing on alternate years. Note, the Aug-Sep 04 hybridization failed and is missing from the dataset
Project description:We present Smart-Seq2 Rolling Circle to Concatemeric Consensus (Smar2C2) for the identification and quantification of transcription start sites. Smar2C2 allows for the identification of upwards of 70 million unique transcription start sites from a single sample with as little as 40 pg of RNA input.