Project description:hormonal progression in prostate cancer fiber model Experiment Overall Design: The LNCaP Hollow Fiber model of prostate cancer was appied. A total of 24 fibers were implanted in each animal, in bundles of eight fibers at three different regions in the animal. Castration of mice was performed At each time point, eight fibers from the same mouse were harvested and total RNA was isolated from LNCaP cells grown inside the fibers using Trizol
Project description:Reduced membrane fouling in an anaerobic electrochemical membrane bioreactors using graphene-coated hollow fiber membranes as the cathode
Project description:Skeletal muscle fiber composition and muscle energetics are not static and change in muscle disease. This study was performed to determine if a mitochondrial myopathy is associated with adjustments in skelatal fiber type composition. These effects of drug induced mitochondrial dysfunction on skeletal muscle fiber type composition were analyzed in an animal model.
Project description:In this study, we performed an RNA-Seq transcriptomic analysis concerning acetic acid bacteria’s acid resistance mechanisms during a continuous and periodical industrial submerged vinegar fermentation process, where the acetic acid concentration fluctuates between ~8% and ~12%
Project description:Traditional gene expression studies extract RNA through destructive cell lysis, restricting analysis to single timepoints and requiring parallel samples. This limits the ability to track transcriptomic changes within the same cell population and poses challenges for experiments with scarce primary samples. To address this, we developed a minimally perturbative, unbiased RNA sampling approach — nano-electroextraction (NEE) — which uses hollow nanostraw membranes with mild electroporation to extract intracellular RNA without compromising cell viability or gene expression.
Project description:Sustained exposure to a young systemic environment rejuvenates aged tissues and promotes stem cell function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint direct effects of circulating factors on specific cell populations. Here we describe a method for the encapsulation of human stem cells in highly diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging independent of physical cellular interactions in-vivo.