Project description:we analyzed pathogen-induced changes in the transcriptome of Vitis vinifera ‘Cabernet sauvignon’ and Vitis aestivalis ‘Norton’ by conducting a large-scale study to measure transcript abundance at 0, 4, 8, 12, 24, and 48 hours post-treatment in conidiospore- and mock-inoculated leaves using Affymetrix GeneChip Vitis vinifera Genome Array Keywords: time course
Project description:Genome-wide expression profiling of Vitis vinifera L. cv. Cabernet Sauvignon (CS) and two rootstocks, Kober 5BB and 41B, exposed to seven days of water deficit or to increasing salinity reaching 120 mM NaCl. Shoot tissue was profiled on the Affymetrix GeneChip Vitis vinifera Genome Array to identify shared and genotype-specific transcriptional responses to the two stresses.
Project description:The influence of temperatures on dormant bud gene expression was analyzed during an eighteen-day period in January 2016 (January 14th to 31st) when daily temperatures ranged between 13 ºC and -15 ºC. This study collection period captured three different natural consecutive temperature events that fluctuated above and below 0 ºC or remained constantly below 0 ºC. Dormant buds were collected from two different wine grapevine cultivars, Cabernet Franc’ and ‘Riesling’. The buds were collected from Ravines Wine Cellar vineyards in Geneva, New York (42.843898, -77.003488. There were two 84-hour diurnal cycles (DC) that included temperatures fluctuating above and below 0 ºC (DC1: Jan 14 to Jan 17; DC2: Jan 24 to Jan 27) (Figure 1, Table 1). In DC1 and DC2, samples were collected 3 times/day, during the dark periods (6:00 and 18:00) and once in the light at warmest part of the day (14:00). The DC1 and DC2 periods bracketed four days (96 h) of constant daily temperatures below 0 ºC (Jan 18 to Jan 22). This constant below 0 ºC period was sampled in the light period only (14:00) (Figure 2, Table 1). Three replicate bud samples, each consisting of 3 buds per replicate were collected, each replicate from a separate vine for Reisling and Cabernet franc. Bud samples were immediately plunged into liquid nitrogen and stored at -80 ºC until RNA extraction.