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RNA-seq analysis CARM1 knockdown effect on MDA-MB-231 cells

ABSTRACT: RNA-seq analysis CARM1 knockdown effect on MDA-MB-231 cells

PROVIDER: PRJNA704560 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13776332.fastq.gz	Fastqsanger.gz
	SRR13776333.fastq.gz	Fastqsanger.gz
	SRR13776334.fastq.gz	Fastqsanger.gz
	SRR13776335.fastq.gz	Fastqsanger.gz

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lncRNA-seq analysis CARM1 knockdown effect on MDA-MB-231 cells

Project description:Previous studies reported that NEAT1 and its partner p54nrb are necessary for CARM1 to associate with paraspeckles and H3R26 methylation. There are also reports that CARM1 is essential for LincGET's role in guiding the ICM lineage in mouse embryos. We speculate that there may be a connection between CARM1 and lncRNA. In order to verify our guess, we conducted lncRNA-seq experiments against CARM1 control or siRNA in MDA-MB-231 cells to explore the lncRNA that may be regulated downstream of CARM1. Here, we showed that CARM1 and HIF1A co-upregulates the expression of MALAT1 in hypoxia pathway to promote carcinogenesis and metastasis in triple-negative breast cancer cells. These results indicate that there is a connection among CARM1, HIF1A and MALAT1 in triple-negative breast cancer.

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