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Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

ABSTRACT: Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

PROVIDER: PRJNA704630 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13777325_1.fastq.gz	Fastqsanger.gz
	SRR13777325_2.fastq.gz	Fastqsanger.gz
	SRR13777326_1.fastq.gz	Fastqsanger.gz
	SRR13777326_2.fastq.gz	Fastqsanger.gz
	SRR13777327_1.fastq.gz	Fastqsanger.gz

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Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [scRNA-seq]

Project description:The early limb bud consists of mesenchymal progenitors (limb progenitors) derived from the lateral plate mesoderm (LPM) that produce most of the tissues of the mature limb bud. The LPM also gives rise to the mesodermal components of the trunk, flank and neck. However, the mesenchymal cells generated at these other axial levels cannot produce the variety of cell types found in the limb bud, nor can they be directed to form a patterned appendage-like structure, even when placed in the context of the signals responsible for organizing the limb bud. Here, by taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28) normally expressed in the early limb bud, that are capable of imparting limb progenitor-like properties to non-limb fibroblasts. Cells reprogrammed by these factors show similar gene expression profiles, and can differentiate into similar cell types, as endogenous limb progenitors. The further addition of Lin41 potentiates proliferation of the reprogrammed cells while suppressing differentiation. These results suggest that these same four key factors may play pivotal roles in the specification of endogenous limb progenitors.

2023-11-15 | GSE167422 | GEO

Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq]

2023-11-15 | GSE167419 | GEO

Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq]

Project description:Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq]

| PRJNA704626 | ENA

Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [scRNA-seq]

Project description:Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [scRNA-seq]

| PRJNA704636 | ENA

Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors.

Project description:The early limb bud consists of mesenchymal limb progenitors derived from the lateral plate mesoderm (LPM). The LPM also gives rise to the mesodermal components of the flank and neck. However, the cells at these other levels cannot produce the variety of cell types found in the limb. Taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28a) normally expressed in the early limb bud and capable of imparting limb progenitor-like properties to mouse non-limb fibroblasts. The reprogrammed cells show similar gene expression profiles and can differentiate into similar cell types as endogenous limb progenitors. The further addition of Lin41 potentiates the proliferation of the reprogrammed cells. These results suggest that these same four factors may play pivotal roles in the specification of endogenous limb progenitors.

| S-EPMC10932627 | biostudies-literature

Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-15 | GSE167423 | GEO

Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

Project description:Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors

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Direct reprogramming of mouse fibroblasts to neural progenitors.

Project description:The simple yet powerful technique of induced pluripotency may eventually supply a wide range of differentiated cells for cell therapy and drug development. However, making the appropriate cells via induced pluripotent stem cells (iPSCs) requires reprogramming of somatic cells and subsequent redifferentiation. Given how arduous and lengthy this process can be, we sought to determine whether it might be possible to convert somatic cells into lineage-specific stem/progenitor cells of another germ layer in one step, bypassing the intermediate pluripotent stage. Here we show that transient induction of the four reprogramming factors (Oct4, Sox2, Klf4, and c-Myc) can efficiently transdifferentiate fibroblasts into functional neural stem/progenitor cells (NPCs) with appropriate signaling inputs. Compared with induced neurons (or iN cells, which are directly converted from fibroblasts), transdifferentiated NPCs have the distinct advantage of being expandable in vitro and retaining the ability to give rise to multiple neuronal subtypes and glial cells. Our results provide a unique paradigm for iPSC-factor-based reprogramming by demonstrating that it can be readily modified to serve as a general platform for transdifferentiation.

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Direct Conversion of Human Fibroblasts into Neuronal Restricted Progenitors

Project description:Neuronal restricted progenitors (NRPs) represent a type of transitional intermediate cells that lie between multipotent neural progenitors (NPs) and terminal differentiated neurons during neurogenesis. These NRPs have the ability to self-renew and differentiate into neurons, but not into glial cells, which is considered as an advantage for cellular therapy of human neurodegenerative diseases. However, difficulty in the extraction of highly purified NPRs from normal nervous tissue prevents further studies and applications. In this study, we reported conversion of human fetal dermal fibroblasts into human induced neuronal restricted progenitors (hiNRPs) in seven days by using just three defined factors: Sox2, c-Myc, and either Brn2 or Brn4. The hiNRPs exhibited distinct neuronal characteristics, including cell morphology, multiple neuronal markers expression, self-renewal capacity, and genome-wide transcriptional profile. Moreover, hiNRPs were able to differentiate into various terminal neurons with functional membrane properties, but not glial cells. Direct generation of hiNRPs from somatic cells will provide a new source of cells for cellular replacement therapy of human neurodegenerative diseases. This is a general expression microarray design (NimbleGen platform). It includes 5 samples.

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Fibroblasts direct differentiation of human breast epithelial progenitors

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