Project description:The purpose of this study was to characterize the gene expression profile of MDA-MB-231 breast cancer cells treated with various SCFA-hexosamine analogs to better understand the role of various modifications to this scaffold. Keywords: SCFA-hexosamine analog comparison
Project description:The effect of three individual SCFA were tested in primary microglial cultured cells, we evaluate microglia transcriptome using Nanostring technology
Project description:The effects of the SCFA crotonate on fungal and host transcriptomes were addressed, following infection of mouse bone marrow-derived macrophages(BMDMs) with Candida albicans.
Project description:The purpose of this study was to characterize the gene expression profile of MDA-MB-231 breast cancer cells treated with various SCFA-hexosamine analogs to better understand the role of various modifications to this scaffold. Experiment Overall Design: In total, six analogs were investigated in this study. For the relatively ""toxic"" C6-SCFA analogs, IC30 was selected to explore initial growth inhibitory and apoptotic gene expression changes. Two independent biological replicates were analyzed for each analog-treated condition. Three independent biological replicates were analyzed for the vehicle-treated control (EtOH).
Project description:Gut microbiota modulate cancer control but how the different bacteria impact tumor-specific CD8+ T cell immunity remains unclear. Here, we identified that spontaneous control of cutaneous melanoma in mice correlated with microbiome-encoded metabolic pathways required for short-chain fatty acid (SCFA) synthesis. Diet-induced enforcement of microbial SCFA production reduced tumor progression and enriched tumor-specific CD8+ T cells in the tumor draining lymph node (tdLN) that lacked features of exhausted T cells. The SCFA butyrate promoted a FOXO1-dependent stemness program in these CD8+ T cells, enhanced the differentiation of CD127+CD8+ T cells and induced immune checkpoint blockade (ICB) responsiveness. Consistent with these experimental studies, metabolic modelling predicted enhanced microbial production of the SCFA butyrate in ICB-responsive melanoma patients and butyrate induced transcriptional features of ICB-responsiveness in CD8+ T cells. Collectively, these data identify the effects of microbial metabolic pathways on tumor-specific CD8+ T cell differentiation as critical components of how the gut microbiome regulates cancer immunity.
Project description:P. bryantii B14 cells were cultivated separately in acetic (Acet), propionic (Prop), butyric (But), iso-butyric (iBut), valeric (Val), iso-valeric (iVal) and 2-methyl butyric acid (2MB) as well as in a mixture of all mentioned short-chain fatty acids (Mix). All 8 treatments were analyzed regarding their proteomes in order to understand the requirements and effects of each SCFA on the metabolism.
