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ABSTRACT: Transcriptional analysis of Panax notoginseng leaves in response to Alternaria panax Whetzel infection

PROVIDER: PRJNA706472 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13853792_1.fastq.gz	Fastqsanger.gz
	SRR13853792_2.fastq.gz	Fastqsanger.gz
	SRR13853793_1.fastq.gz	Fastqsanger.gz
	SRR13853793_2.fastq.gz	Fastqsanger.gz
	SRR13853794_1.fastq.gz	Fastqsanger.gz

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Project description:The total RNA were extracted from tissues of roots from several plants of Panax notoginseng under CK and Cd stress treatment by using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The purified PCR product was sequenced using Illumina Genome Analyzer II. The qualified reads were used to study of Panax notoginseng transcriptome under CK and Cd stress treatment.

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MS data of tryptic peptides obtained through activity-guided protein purification

Project description:Protein band obtained from the Panax notoginseng leaves homogenate following an activity-guided protein-purification procedure, that showed regioselective ginsenoside hydrolysis activity was digested with trypsin and analyzed by Nano LC-LTQ Orbitrap MS to construct a peptide library of accessible amino acid sequences. Subsequent mutual sequence alignment analysis between the peptide and transcript libraries identified three candidate genes.

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