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Megakaryocyte derived immunoregulatory cells regulate host-defense immunity against bacterial pathogens

ABSTRACT: Megakaryocyte derived immunoregulatory cells regulate host-defense immunity against bacterial pathogens

PROVIDER: PRJNA706584 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13856134.fastq.gz	Fastqsanger.gz
	SRR13856135.fastq.gz	Fastqsanger.gz
	SRR13856136.fastq.gz	Fastqsanger.gz
	SRR13856137.fastq.gz	Fastqsanger.gz
	SRR13856138.fastq.gz	Fastqsanger.gz

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Megakaryocyte derived immunoregulatory cells regulate host-defense immunity against bacterial pathogens

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Project description:Mast cells (MCs) are critical mediators of inflammation; however, their microbicidal activity against invading pathogens remains largely unknown. Here, we describe a nonpreviously reported antibacterial mechanism used by MCs against Coxiella burnetii, the agent of Q fever. We show that C. burnetii interaction with MCs does not result in bacterial uptake but rather induces the formation of extracellular actin filaments named cytonemes. MC cytonemes express cathelicidin and neutrophil elastase and mediate the capture and destruction of entrapped bacteria. We provide evidence that MC cytoneme formation and microbicidal activity are dependent on the cooperation of the scavenger receptor CD36 and Toll-like receptor 4. Taken together, our results suggest that MCs use an extracellular sophisticated mechanism of defense to eliminate intracellular pathogens, such as C. burnetii, before their entry into host cells.

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