Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq data for ex vivo Naïve CD8+ T cells and TCR activated 24hr in the presence or absence of PI3Kp110δ inhibitor

ABSTRACT: RNAseq data for ex vivo Naïve CD8+ T cells and TCR activated 24hr in the presence or absence of PI3Kp110δ inhibitor

PROVIDER: PRJNA716601 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14044884_1.fastq.gz	Fastqsanger.gz
	SRR14044884_2.fastq.gz	Fastqsanger.gz
	SRR14044885_1.fastq.gz	Fastqsanger.gz
	SRR14044885_2.fastq.gz	Fastqsanger.gz
	SRR14044886_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

RNAseq data for ex vivo Naïve CD8+ T cells and TCR activated 24hr in the presence or absence of PI3Kp110δ inhibitor

Project description:Phosphatidylinositol-3-kinase p110 delta (PI3Kp110δ) is pivotal for CD8+ T cell immune responses. To inform how PI3Kp110δ directs CD8+ T cell fate the current study focuses on PI3Kp110δ controlled transcriptional programs and reveals how PI3Kp110δ selectively induces and represses expression of key genes that create a cytotoxic T cell (CTL). The data identify differences in PI3Kp110δ regulated transcriptional programs between naïve and effector cytotoxic T cells including differential control of cytolytic effector molecules, costimulatory receptors and the critical inhibitory receptors CTLA4 and SLAMF6. However, common to both naïve and effector cells is PI3Kp110δ control of the production of chemokines and cytokines that orchestrate communication between the adaptive and innate immune system. The study provides a comprehensive resource for understanding how PI3Kp110δ uses multiple processes mediated by Protein Kinase B/AKT, FOXO1 dependent and independent mechanisms and mitogen-activated protein kinases (MAPK) to direct CD8+ T cell fate.

2021-06-16 | GSE169436 | GEO

Total RNA: naïve ex vivo mouse OT-1 T lymphocytes, WT vs Lck-/- (OFF)

Project description:This experiment aims to study the transcriptome of naïve T cells at the steady state. A T cell receptor (TCR) transgenic mouse model (OT-1) posessing a homogenous alpha-beta CD8 T lymphocyte population was used, and cells with intact TCR signalling pathways (WT) were compared to those with defective signal transduction (Lck-/-, OFF). Naïve (CD8b+, CD44low) ex vivo T cells were isolated from the superficial cervicals, axillary, brachial, mesenteric, and inguinal lymph nodes. Total RNA was extracted from the cells, labelled with Cy3, and hybridised onto an Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray.

2016-11-30 | GSE84855 | GEO

Role of PKD2 in TCR-induced transcriptional reprogramming of naïve T cells

Project description:Comparison of transcriptional profile of TCR stimulated P14-TCR wild-type and P14-PKD2 null murine lymph node cells Lymph node cells from 3 biological replicates (3 wild-type and 3 PKD2 null mice) were isolated and left unstimulated or stimulated for 4 hours with antigenic peptide (gp33-41) prior to RNA extraction and hybridization to Affymetrix microarray.

2012-02-29 | E-GEOD-33942 | biostudies-arrayexpress

Gene expression profile at 24hr after ANIT administration

Project description:Gene expression profile at 24hr after alpha-naphtylisothiocyanate administration. Hybridization was carried out four times including color swap. Keywords: repeat sample

2003-12-13 | GSE894 | GEO

TCR

Project description:TCR

2021-07-15 | OEX00013711 | NODE

Role of PKD2 in TCR-induced transcriptional reprogramming of naïve T cells

Project description:Comparison of transcriptional profile of TCR stimulated P14-TCR wild-type and P14-PKD2 null murine lymph node cells

2012-02-29 | GSE33942 | GEO

Expression data from CD4+ naïve T cells under Treg-inducing condition in the presence or absence of butyrate

Project description:Commensal bacteria shape the gut immune system. Colonization bacteria increase the frequency of regulatory T cells, however, the molecular mechanisms are not yet known. To reveal the mechanism, we isolated naïve CD4+ T cells from the spleen of C57BL/6 mice and cultured the cells under Treg-inducing condition culture in the presence or absence of butyrate, a metabolite produced by commensal bacteria.

2015-06-12 | GSE48045 | GEO

Expression data from CD4+ Naïve T cells under Treg-inducing condition in the presence or absence of butyrate

Project description:Commensal bacteria shapes gut immune system. Colonization bacteria increase the frequency of regulatory T cells, however, the molecular mechanisms has not yet been unknown. To reveal the mechanism, we isolated Naïve CD4+ T cells from spleen of C57BL/6 mice and cultured the cells under Treg-inducing condition culture in the presence or absence of butyrate, a metabolite produced by commensal bacteria.

2013-11-20 | GSE49619 | GEO

RNAseq data from unstimulated or TCR retriggered (4hr) cytotoxic T lymphocytes (CTL) in the presence or absence of PI3Kp110δ or MEK inhibitors

Project description:RNAseq data from unstimulated or TCR retriggered (4hr) cytotoxic T lymphocytes (CTL) in the presence or absence of PI3Kp110δ or MEK inhibitors

| PRJNA716628 | ENA

Peptide Driven Identification of TCRs (PDI-TCR) reveals dynamics and phenotypes of CD4 T cells in tuberculosis [TCR-Seq]

Project description:We developed the Peptide-Driven Identification of TCRs (PDI-TCR), an approach that leverages antigen-specific peptide pools, bulk TCR sequencing, and statistical analyses to pinpoint antigen-specific TCRs. We discerned antigen-specific TCRs within a group of active tuberculosis (ATB) patients and individuals with a positive Interferon-γ release assay (IGRA+). We found Mtb-specific effectors to have an HLA-DR+ Th1* phenotype, and to be exclusive to active disease. Conversely, HLA-DR- Mtb-specific T cells appeared in Th1 cells in both ATB and IGRA+ cohorts. We also identified Cross-reactive T cells, that were abundant ex vivo regardless of disease state having a cytotoxic phenotype.

2025-07-07 | GSE301489 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data