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DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and plant development

ABSTRACT: DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and plant development

PROVIDER: PRJNA716808 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14056759_1.fastq.gz	Fastqsanger.gz
	SRR14056759_2.fastq.gz	Fastqsanger.gz
	SRR14056760_1.fastq.gz	Fastqsanger.gz
	SRR14056760_2.fastq.gz	Fastqsanger.gz
	SRR14056761_1.fastq.gz	Fastqsanger.gz

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DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and plant development

Project description:A contribution of DNA methylation to defense against invading nucleic acids and maintenance of genome integrity is uncontested; however, the relevance of this epigenetic mark for genome-wide gene regulation and plant development is unclear. Here, we knocked out all five known DNA methyltransferases in Arabidopsis, generating DNA methylation-free plants. This quintuple mutant exhibits a suite of unique and extreme developmental defects, unequivocally demonstrating that DNA methylation is essential for normal plant development. We show that CG methylation or non-CG methylation alone or jointly control multiple biological processes, including pavement cell shape, endoreduplication, cell death, flowering, trichome morphology, vasculature and meristem development, and root cell fate determination. Moreover, we found that DNA methylation has a strong dose-dependent effect in the regulation of gene expression and the repression of transposable element movement. Thus, our results demonstrate crucial roles of DNA methylation in orchestrating plant development and provide new insights into the function of this epigenetic mark in regulating gene expression in higher eukaryotes.

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Project description:A contribution of DNA methylation to defense against invading nucleic acids and maintenance of genome integrity is uncontested; however, our understanding of the extent of involvement of this epigenetic mark in genome-wide gene regulation and plant developmental control is incomplete. Here, we knock out all five known DNA methyltransferases in Arabidopsis, generating DNA methylation-free plants. This quintuple mutant exhibits a suite of developmental defects, unequivocally demonstrating that DNA methylation is essential for multiple aspects of plant development. We show that CG methylation and non-CG methylation are required for a plethora of biological processes, including pavement cell shape, endoreduplication, cell death, flowering, trichome morphology, vasculature and meristem development, and root cell fate determination. Moreover, we find that DNA methylation has a strong dose-dependent effect on gene expression and repression of transposable elements. Taken together, our results demonstrate that DNA methylation is dispensable for Arabidopsis survival but essential for the proper regulation of multiple biological processes.

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