Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Root transcriptome of CLE3 overexpressing lines and clv1-15 mutant lines

ABSTRACT: Root transcriptome of CLE3 overexpressing lines and clv1-15 mutant lines

PROVIDER: PRJNA717784 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Root transcriptome of CLE3 overexpressing lines and clv1-15 mutant lines

Project description:Nitrogen availability significantly impacts the development of plant root system architecture as plants must optimize growth for efficient acquisition of this nutrient. A signaling pathway involving the CLE family of small signaling peptides and the CLV1 leucine-rich repeat receptor kinase has been shown to repress lateral root development when plants are undergoing nitrogen starvation. Targets of this pathway remain unstudied and may be implicated in the coordination of N-responsive root growth. We obtained the clv1-15 mutant in Landsberg erecta (Ler) background and have generated transgenic lines overexpressing CLE3 in Columbia-0 (Col-0) background for further analysis. To identify downstream components of CLE-CLV1 signaling, we analyzed the transcriptome profiles of CLE3 overexpressing lines and the clv1-15 mutant.

2025-03-26 | GSE169744 | GEO

Analysis of differential gene expression in mutant and overexpressing lines of DNE1

Project description:Analysis of differential gene expression in mutant and overexpressing lines of DNE1

| PRJNA655682 | ENA

Transcript profiling of hairy root lines overexpressing MtPAR

Project description:Hairy root lines over-expressing MtPAR using a 35S promoter compared with hairy lines over-expressing GUS gene. Hairy roots were generated in vitro using leaf explants from Medicago A17. The goal of this experiment is to prove that ectopic expression expression of MtPAR is sufficient to induce tannin biosynthesis

2012-01-31 | E-MEXP-3485 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type, arabidillo-1/2 mutant and Arabidillo-1 overexpressing lines to identify targets of Arabidillo

Project description:Arabidopsis has two genes, Arabidillo-1 and -2, related to animal Armadillo/ beta-catenin (Coates, 2003). Armadillo/beta-catenin directly activates the expression of developmental and cell proliferation genes, and also independently regulates cell-cell adhesion. Arabidillo proteins are nuclear and promote lateral root development. We aim to identify candidate Arabidillo target genes by comparing the transcriptomes of wild type, arabidillo-1/2 mutant and Arabidillo-1 overexpressing lines. The experiment will compare Col-0 (3 slides) with a 35S::Arabidillo-1-YFP overexpressing line (3 slides) and Col-3 with the arabidillo-1/2 mutant (3 slides). Each RNA prep will be from plate-grown 7-day old seedlings. Reference: Coates, JC (2003) Armadillo repeat proteins: beyond the animal kingdom? Trends in Cell Biology 13 p.463-471; Experimenter name: Juliet Coates; Experimenter phone: 0121 414 5481; Experimenter institute: University of Birmingham; Experimenter address: School of Biosciences; Experimenter address: Birmingham; Experimenter zip/postal_code: B15 2TT; Experimenter country: UK Experiment Overall Design: 12 samples were used in this experiment

2008-06-15 | E-GEOD-6826 | biostudies-arrayexpress

Overexpression of OsbHLH044 transcription factor controls a negative regulatory module associated with plant cell death and stress inhibition in rice crown root development

Project description:Crown roots constitute the main part of the rice root system. Several key genes involved in crown root initiation and development have been identified by genetics and functional genomics approaches. The CROWN ROOTLESS1 (CRL1) transcription factor is necessary for crown root initiation in rice. The basic helix-loop-helix 044 (OsbHLH044) transcription factor was identified as a CRL1-regulated gene. In this study, we aimed to determine OsbHLH044-regulated genes, with transcriptome analysis of OsbHLH44 overexpressing plants in crl1 mutant background. We analysed the transcriptional profile of overexpressing lines stem bases in comparison with corresponding null segregant sister control lines. Affymetrix microarrays were processed in the Microarray Core Facility “Transcriptome“ of the Institute in Regenerative Medicine and Biotherapy, CHU de Montpellier-INSERM-UM Montpellier, http://irmb.chu-montpellier.fr/ .

2020-03-20 | GSE147200 | GEO

RNA-seq of HEK293 cells overexpressing NOCT ∆(2-15)-3F with controls overexpressing GST-3F

Project description:RNA-seq of HEK293 cells overexpressing NOCT ∆(2-15)-3F with controls overexpressing GST-3F

| PRJNA598199 | ENA

Comparison of the root tip transcriptome of 2 pearl millet lines with contrasted primary root growth

Project description:We analysed global gene expression in the primary root tip of 2 pearl millet (Pennisetum glaucum) inbred lines with high (line 249) and low (line 337) primary root growth using RNAseq. The objective was to identify genes potentially associated with changes in root growth.

2021-10-10 | GSE185517 | GEO

Mouse embryonic stem cells (mESCs) and neurons overexpressing DNMT1 and DNMT1 Y495C mutant form

Project description:Genome wide DNA methylation profiling of mESCs and neurons overexpressing DNMT1 and DNMT1 Y495C mutant form. The Infinium Mouse methylation Beadchip was used to obtain DNA methylation profiles across approximately > 285k methylation sites in DNA samples of mESCs and neurons. Samples included wild-type cell line (R1) and transgenic cell lines (R1wtDnmt1 and R1Dnmt1Y495C).

2023-12-01 | GSE241544 | GEO

Root transcriptome of the tsn1 tsn2 double mutant

Project description:au06-03_tsn_fj - tsn_fj - Root transcriptome of the tsn1 tsn2 double mutant - Comparison of a tsn1 tsn2 double mutant line (A10.9.3- or A13.3.11-) and an isogenic line complemented by TSN2 (A10.9.5) or TSN1 (A13.3.2) Keywords: gene knock in (transgenic),gene knock out

2009-04-08 | GSE15559 | GEO

Bulk sequencing of MYCN-overexpressing chicken retinal cell lines

Project description:Bulk sequencing of MYCN-overexpressing chicken retinal cell lines

| PRJNA818671 | ENA