Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Comparative Phylotranscriptomics Reveals Putative Sex Determining Genes in Bivalvia

PROVIDER: PRJNA719382 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14135100.fastq.gz	Fastqsanger.gz
	SRR14135101.fastq.gz	Fastqsanger.gz
	SRR14135102.fastq.gz	Fastqsanger.gz
	SRR14135103.fastq.gz	Fastqsanger.gz
	SRR14135104.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Utterbackia imbecillis

Project description:Putative mitochondrial sex determination in the Bivalvia: insights from a hybrid transcriptome assembly in freshwater mussels

| PRJNA528115 | ENA

CBX2 is required to stabilize the testis pathway by repressing Wnt signaling

Project description:Purpose: Determine whether sex-determining genes are bivalent at the bipotential stage, poised between the testis and ovary fate, and whether H3K4me3 and H3K27me3 resolve into sex-specific patterns after sex determination, contributing to the canalization and stabilization of either the testis or ovary fate. Methods: XX and XY supporting cells of the gonad were FACS-purified before sex determination (at E10.5) and after sex determination (at E13.5), and submitted to ChIP-seq for H3K4me3, H3K27me3 and H3 as a means to normalize across cell populations. Results: We found that key sex-determining genes are bivalent at the bipotential stage. Genes that are upregulated affter sex determination are stripped of their repressive H3K27me3 mark, whereas repressed genes that promote the alternate pathway remain bivalent even after sex determination.

2019-05-07 | GSE130749 | GEO

The male determining M-factor of the global disease vector Musca domestica is a paralog of the generic splice factor CWC22

Project description:Sex determination mechanisms are bewilderingly diverse. A cascade of genes with hierarchical regulation characterizes sex determination pathways in insects. How such pathways evolve is poorly understood, partly due to a lack of comparative data. Houseflies are well known for their polymorphic sex determination with populations carrying a dominant male determiner M on the Y chromosome or any of the five autosomes. We identified the male determining gene Mdm responsible for splicing regulation of the key switch transformer by exploiting the existence of housefly populations with different sex determination mechanisms. We demonstrate that Mdm originated from duplication of CWC22/nucampholin, a generic and essential splicing regulator across Metazoans with a crucial role in exon-junction complex assembly and non-sense mediated decay. We show that strains with the M-locus on the Y and on different autosomes carry multiple copies of Mdm indicating that the same male determiner translocated to different genomic sites in the genome. We found that embryonic RNAi-based silencing of Mdm leads to differentiation of ovaries in males, while targeted Mdm disruption with CRISPR/CAS-9 resulted in complete sex reversal to fertile females. Our study reveals how a duplicate of a gene with a general splice-regulation role during development can be recruited to serve a specific function in the determination of male sex. Mdm appears to be unique to the housefly representing a compelling example for the plasticity at the instructive level of sex determination hierarchies.

2017-05-12 | E-MTAB-5080 | biostudies-arrayexpress

Asparagales

Project description:Phylotranscriptomics reveals the phylogeny of Asparagales

| PRJNA1107703 | ENA

A Y-chromosome-encoded small RNA acts as sex determinant in persimmons

Project description:In plants, multiple lineages have evolved sex chromosomes independently, providing a powerful comparative framework, but few specific determinants controlling the expression of a specific sex have been identified. We investigated sex-determinants in Caucasian persimmon, Diospyros lotus, a dioecious plant with heterogametic males (XY). Male-specific short nucleotide sequences were used to define a male determining region. A combination of transcriptomics and evolutionary approaches detected a Y-specific sex-determinant candidate, OGI, that displays male-specific conservation among Diospyros species. OGI encodes a small RNA targeting the autosomal MeGI gene, a homeodomain transcription factor regulating anther fertility in dosage-dependent fashion. This identification of a feminizing gene suppressed by a Y-chromosome-encoded small RNA contributes to our understanding of the evolution of sex chromosome systems in higher plants.

2014-11-04 | GSE61386 | GEO

Comparative transcriptome analysis of testes and ovaries reveals sex-biased genes and pathways in zebrafish

Project description:Comparative transcriptome analysis of testes and ovaries reveals sex-biased genes and pathways in zebrafish

| PRJNA944944 | ENA

Project description:Sex determining region turnovers of Hippocampus species

| PRJNA736169 | ENA

Oryza sativa

Project description:OsTOC1 putative target genes

| PRJNA1057527 | ENA

Transcriptomic responses to environmental temperature by turtles with thermosensitive and genotypic sex determination assessed by RNAseq inform the genetic architecture of embryonic gonadal development

Project description:We report the expression profiles of putative genes involved in temperature-dependent sex determination across multiple developmental stages in turtles, and contrast this data with equivalent stages in turtles with sex chromosomes

2018-02-14 | GSE94871 | GEO

Takifugu snyderi

Project description:Identification of sex determining region of Takifugu snyderi

| PRJDB12435 | ENA