Project description:ChIP-seq data characterizing the occupancy of TFAM over the mitochondrial and nuclear genomes in HeLa cells. Characterization of mitochondrial and nuclear genome-wide TFAM binding in HeLa cells
Project description:We report mitochondrial genome (mtDNA) sequences in purified mouse muscle stem cells at different ages. This study identifies changes in the mitochondrial genome of muscle stem cells during aging.
Project description:To better understand genome coordination and OXPHOS recovery during mitochondrial dysfunction, we examined ATFS-1, a transcription factor that regulates mitochondria-to nuclear communication during the mitochondrial UPR, via ChIP-sequencing. Wildtype worms treated spg-7(RNAi) are analyzed in the presence and absence of ATFS-1 antibody to identify ATFS-1 targets. Individual samples were analyzed. Wildtype worms treated spg-7(RNAi) in the absence of antibody is used as a control.
Project description:The aim is to investigate the impact of prostate and colorectal cancer on mitochondrial quantity and quality along with muscle mass and function and whether this can be modified through the use of a home-based short-term exercise training program.
The investigators aim to recruit participants awaiting curative surgery for colorectal and prostate cancer and to assess the variation in baseline mitochondrial activity between them.
Participants from both cancer types will then carry out a 4 week home exercise program, this will be randomly allocated to either resistance-based or high-intensity interval training based. Participants will then be re-assessed on the day of their planned surgical procedure to assess the changes effected by the training program.
The investigators hypothesize that there will be variation in mitochondrial activity linked to muscle mass across the two cancer types and that home-based exercise programs have the ability to improve mitochondrial activity along with muscle mass.
Project description:Mitochondria are critical sites for oxidative respiration and material exchange within mammalian cells. The mitochondrial genome encodes 22 types of tRNA and is responsible for the transcription and translation of 13 proteins involved in the oxidative respiratory chain. During the translation process, the tRNAs encoded by the mitochondrial genome decode 64 types of codons to ensure proper protein translation. The wobble hypothesis posits that mitochondrial tRNAs recognize codons through non-strict base pairing and specific modifications on the anticodon, thereby facilitating accurate decoding. However, it remains unclear whether nuclear-encoded tRNAs can enter the mitochondria and participate in mitochondrial translation.Existing studies have demonstrated that in lower organisms, the import of tRNAs into mitochondria requires the assistance of several cytosolic proteins to guide them to the mitochondrial surface, where they form RNA import complexes (RICs) with mitochondrial inner and outer membrane proteins