Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ENampt, CRH and Il-6 influence on gene expression of rat primary corticotropes.

ABSTRACT: eNampt, CRH and Il-6 influence on gene expression of rat primary corticotropes.

PROVIDER: PRJNA723869 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

eNampt, CRH and Il-6 influence on gene expression of rat primary corticotropes.

Project description:ACTH is known to be secreted from pituitary cortictropes after CRH and Il-6 stimulation. Recenetly it was proven that eNampt also stimulates ACTH secretion from rat pituitary gland. We used microarrays to detail the global programme of gene expression in isolated rat pituitary corticotropes, 24h after administration of eNampt, CRH and Il-6.

2021-05-31 | GSE173142 | GEO

Primary Trophoblasts treated with Corticotropin Releasing Hormone (CRH)

Project description:Primary trophoblast cells were treated with corticotropin releasing hormone (CRH) and then subjected to RNA extraction and sequencing.

2025-03-13 | GSE279747 | GEO

Single-nuclei RNA-seq (snRNA-seq) of postnatal hypothalamus of CRH-IRESCre (CRH-Cre) mice using a retrograde Connect-seq approach

Project description:We generated a single nuclei RNA-seq (snRNA-seq) dataset derived from the postnatal hypothalamus of CRH-IRESCre (CRH-Cre) mice using a retrograde Connect-seq approach.

2023-08-19 | GSE240765 | GEO

Primary Trophoblasts treated with Corticotropin Releasing Hormone (CRH)

Project description:Primary Trophoblasts treated with Corticotropin Releasing Hormone (CRH)

| PRJNA1174194 | ENA

Cell-Specific Transcriptomic Analysis of CRH+ cells Identifies Unique Cellular Responses to Chronic Stress

Project description:Rationale: A previous transcriptome meta-analysis revealed significantly lower levels of corticotropin-releasing hormone (CRH) mRNA in corticolimbic brain regions in major depressive disorder (MDD) subjects. Rodent studies show that cortical CRH is mostly expressed in GABAergic neurons; however, the characteristic features of CRH+ cells in human brain cortex and their association with MDD are largely unknown. Methods: Subgenual anterior cingulate cortex (sgACC) of human subjects without brain disorders were labeled using fluorescent in situ hybridization (FISH) for CRH and markers of excitatory (SLC17A7), inhibitory (GAD1) neurons, as well as markers of other interneuron subpopulations (PVALB, SST, VIP). MDD-associated changes in CRH+ cell density and cellular CRH expression (n=6/group) were analyzed. RNA-sequencing was performed on sgACC CRH+ neurons from comparison and MDD subjects (n=6/group), and analyzed for group differences. Results: About 80% of CRH+ cells were GABAergic and 17.5% were glutamatergic. CRH+ GABAergic neurons co-expressed VIP (52%), SST (7%), or PVALB (7%). MDD subjects displayed lower CRH mRNA levels in GABAergic neurons relative to comparison subjects without changes in cell density. CRH+ neurons show transcriptomic profile suggesting lower excitability and less GABA release and reuptake. Further analyses suggested that these molecular changes are not mediated by altered glucocorticoid feedback and potentially occur downstream for a common modulator of neurotrophic function. Summary: CRH+ cells in human sgACC are a heterogeneous population of GABAergic neurons, although largely co-expressing VIP. MDD is associated with reduced markers of inhibitory function of CRH+ neurons.

2022-02-22 | GSE193417 | GEO

Genome-wide translational profiling of amygdala Crh-expressing

Project description:Here we translationally profiled the transcriptome of Crh-expressing neurons (Crh neurons) within the CeA following fear conditioning (FC) and fear extinction (EXT) in mice using translating ribosome affinity purification (TRAP) followed by RNA sequencing. A tone alone group (TA) was used as a control group.

2020-09-05 | GSE157021 | GEO

Transcriptional effect of CRH on MCF7 estrogen receptor signalling

2013-07-02 | GSE47958 | GEO

CRH

Project description:Oryctolagus cuniculus CRH, Oryctolagus cuniculus corticotropin releasing hormone (CRH), mRNA. [Source:RefSeq mRNA;Acc:NM_001199007], is expressed in 1 baseline experiment(s);

| ENSOCUG00000023819 | ExpressionAtlas

Transcriptional effect of CRH on MCF7 estrogen receptor signalling

Project description:MCF7 cells were stimulated with vehicle or 100nM corticotropin releasing hormone (CRH) for 24h. The effect of CRH on the expression of genes relevant to estrogen signalling was investigated by using the Human Estrogen Receptor Signaling RTM-BM-2 ProfilerM-bM-^DM-" PCR Array (SABioscience Corp). qPCR gene expression profiling. MCF7 cells were treated separately in triplicate. Equal amount total RNA was processed further for gene expression analysis.

2013-07-02 | E-GEOD-47958 | biostudies-arrayexpress

Expression profiling of 1-day old C. elegans adult circ-crh-1(syb385), crh-1(n3315) versus wild-type

Project description:poly(A)-selected mRNA-seq of circ-crh1(syb385), crh-1(n3315) versus wild-type (N2)

2022-01-27 | GSE190124 | GEO