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Single Cell RNA for in vitro neural tube

ABSTRACT: Single Cell RNA for in vitro neural tube

PROVIDER: PRJNA725826 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14343844_1.fastq.gz	Fastqsanger.gz
	SRR14343844_2.fastq.gz	Fastqsanger.gz
	SRR14343845_1.fastq.gz	Fastqsanger.gz
	SRR14343845_2.fastq.gz	Fastqsanger.gz
	SRR14343846_1.fastq.gz	Fastqsanger.gz

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Identification of transcriptome of mouse non-neural ectoderm during nueral tube closure and gene enrichment compared to remaining neural tube tissue

Project description:The process of neural tube closure is a highly complex morphogenetic event that results in the generation of the primordial central nervous system. During formation of the neural tube, the non-neural ectoderm separates from the neighboring neural ectoderm and forms a single layer epithelial sheet that overlies the closed neural tube. Previous work has shown that the non-neural ectoderm is necessary for proper cranial neural tube closure, however little is known about this cell population at the molecular level or how the non-neural ectoderm contributes to neural tube closure. In this study, we used a mouse genetic system to fluorescently label the non-neural ectoderm cells and FACS sorted these cells away from the other cell populations in the neural tube. We performed high throughput RNA-sequencing to identify the transcriptome of the non-neural ectoderm and compared the gene expression profile of non-neural ectoderm cells to the remaining population of cells within the neural tube in order to identify which genes are enriched within the non-neural ectoderm. This analysis provides a clue as to which underlying molecular processes may be important for non-neural ectoderm function during neural tube closure.

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