Project description:Small nucleolar RNAs (snoRNA) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs. But we found that knock down of a C/D box snoRNA, Bm-15, can induce apoptosis of insect Spodoptera frugiperda Sf9 cells. For the genome sequence of Spodoptera frugiperda is incomplete, here with the de novo sequencing method, transcriptome of Spodoptera frugiperda cell line Sf9 were sequenced after being transfected with overexpression vector and repression probes of snoRNA Bm-15. Results showed that 21 apoptosis-related genes were up-regulated upon Bm-15 inhibition and down-regulated with Bm-15 overexpression.
Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series
Project description:We report the application of whole transcriptome sequencing technology for high-throughput profiling of coding and non-coding RNAs associated with Spodoptera frugiperda feeding in Zea mays. 4,366 mRNAs and 233 lncRNAs were differentially expressed during Spodoptera frugiperda feeding in Zea mays. Our data contribute to the understanding of the function of coding and non-coding RNAs in the regulation of plant-insect interactions.
Project description:Brassinosteroids (BRs) are plant steroid hormones sensed at the cell surface by the membrane receptor kinase BRI1. Activation of BRI1 leads to the dephosphorylation of BZR1/BES1 transcription factors through inactivation of the glycogen synthase kinase 3 BIN2. Here, the in vitro phosphorylation sites of the isolated BSU1 Kelch domain (residues 11-351), phosphatase domain (residues 421-793) and of full-length BSU1 (residues 1-793) recombinantly expressed in baculovirus-infected Spodoptera frugiperda cells were identified in isolation (labelled ctrl) or after incubation with the GSK3 kinase BIN2, expressed as a thioredoxin A fusion protein in E. coli. Reactions were performed in the presence of 100 micromolar ATP and 1 mM MgCl2 for 1:30 h at room temperature.
Project description:Peptide fingerprinting to verify the efficacy of phosphatase inhibitor beads (PIBs) for capturing Spodoptera frugiperda (Sf9) phosphatases of the PP2A family.
Project description:The ARAL-DIA proteome was tested on the third instar larvae of Spodoptera frugiperda with and without resistance to chlorobenamide to obtain different databases, which is important for the study of Spodoptera frugiperda resistance
Project description:In this study, NanoString technology gene expression quantification platform was used to study the expression of toxin genes causing infections from Bacteria (Photorhabdus and Xenorhabdus), Nematode (H.indica, S.riobrave , S.carpocapsae) specific genes for detection and Immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella). The study revealed the expression of different immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella) and helped in understanding the trend of expression of gene in the samples from the healthy condition to the death stage. Variations in gene expression were seen as per the expectation.