Project description:Human aortic endothelial cells were stimulated by lysophosphatidylcholine (LPC) (10μM) with or without interleukin 35 (IL-35) (10ng/mL) or IL-10 (10ng/mL) for 18 hours. Total RNAs were extracted from samples, then mRNA and non-coding RNAs were enriched by removing rRNA from the total RNA. The library was sequenced by Illumina HiSeq4000 using PE100 strategy and the reads were mapped to the human hg19 reference genome.

2019-10-28 | E-MTAB-6604 | biostudies-arrayexpress

Single nucleus RNA-seq of subcortical white matter from lysophosphatidylcholine (LPC) and cuprizone toxicity mouse models

Project description:We compared the transcriptional changes in the cells of the corpus callosum, focusing on the oligodendrocyte and microglia, between LPC and cuprizone mediate demyelination. We further compared the transcriptional phenotypes to human MS patient samples. We identified distinct disease-associated oligodendrocyte states with shared pathological changes to MS, and observed a common but altered remyelination state between the models. Microglia response to demyelination is highly conserved, but human MS-associated microglia exhibit significantly more heterogeneity than we found in the mouse models. (doi: https://doi.org/10.1101/2025.03.24.645058)

2025-04-09 | GSE293850 | GEO

HK-2 cells: control and Substance P (SP)-treated

Project description:NK-1R axis in HK-2 cells by treated HK-2-overexpressed NK-1R cells with or without SP.

2023-03-20 | GSE216743 | GEO

Single nucleus RNA-seq of subcortical white matter from lysophosphatidylcholine (LPC) and cuprizone toxicity mouse models

Project description:Single nucleus RNA-seq of subcortical white matter from lysophosphatidylcholine (LPC) and cuprizone toxicity mouse models

| PRJNA1246592 | ENA

RNA sequencing of paVICs exposed to LPC and dantrolene

Project description:To investigate the gene expression changes due to LPC-induced calcific nodule formation in porcine aortic valve interstitial cells (paVICs). We then examined differential gene expression due to treatment with dantrolene, which inhibits calcific nodule formation, with and without LPC after 24 hours of exposure.

2023-03-30 | GSE227229 | GEO

Patient-derived HK-374 glioblastoma cells treated with radiation and ONC201

Project description:RNASeq results for HK-374 treated with DMSO, radiation, ONC201 or radiation plus ONC201

2020-07-08 | GSE153982 | GEO

Project description:Drosophila melanogaster Hk, Hyperkinetic, is differentially expressed in 8 experiment(s);

| FBgn0263220 | ExpressionAtlas