Project description:To understand differences of gene expression profiles between Francisella strains RNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000. qRT–PCR validation was performed using SYBR Green assays. Our study represents the first detailed differential transcriptomic analysis of Francisella strains , with biologic replicates, generated by RNA-seq technology.
Project description:Perivascular adipose (PVAT and subcutaneous adipose tissue (SubQ) from donors undergoing mitral valve repair/replacement (VR) and coronary artery bypass graft (CABG) surgeries were analyzed. There are 3 SWATH data sets. The first contains CABP PVAT, CABG SubQ, VR PVAT, and VR SubQ. The second includes only CABG PVAT from donors with and without diabetes. The third set includes CABG PVAT and SubQ and VR PVAT. Additionally, there are multiple reaction monitoring data sets comparing CABG PVAT with and without diabetes, CABG SubQ with and without diabetes, CABG PVAT to CABG SubQ both without diabetes, CABG PVAT to CABG SubQ both with diabetes, and CABG PVAT without diabetes to VR PVAT without diabetes.