Project description:UV-induced pyrimidine-pyrimidone (6-4) photoproducts (6-4PPs) and atypical thymine-adenine (TA) photoproducts were mapped in normal human skin fibroblasts following 500J/m2 of UVC irradiation and in isolated human genomic DNA (naked DNA control) irradiated with 400J/m2 of UVC irradiation. 6-4PPs and TA-PPs were mapped across the human genome using the UVDE-HS-seq method
Project description:Here we have used a novel high-throughput sequencing method known as UVDE-seq to map the formation of non-CPD lesions at single nucleotide resolution across the yeast genome in wild-type and rad16 mutant cells, which are deficient in GG-NER.
Project description:We used a novel high-throughput sequencing method known as UVDE-HS-seq to map the formation of UV-induced 6-4 photoproducts (6-4PP) and atypical thymidine-adenine (TA) photoproducts at single nucleotide resolution across the yeast genome in wild-type cells. We also analyzed 6-4PP and TA photoproducts in UV-irradiated yeast genomic DNA (i.e., naked DNA).
Project description:UVDE-HS-seq map of 6-4PP and atypical TA photoproducts in UV-irradiated normal human skin fibroblasts and UV-irradiated genomic DNA
Project description:We used a novel high-throughput sequencing method known as UVDE-seq to map the formation of UV-induced 6-4 photoproducts (6-4PP) and atypical thymidine-adenine (TA) photoproducts at single nucleotide resolution across the yeast genome in wild-type and rad16 mutant cells, which are deficient in GG-NER. We also analyzed 6-4PP and TA photoproducts in UV-irradiated yeast genomic DNA (i.e., naked DNA).
Project description:CPD-capture-seq map of CPD formation in UV-irradiated primary melanocytes, normal human skin fibroblasts and UV-irradiated genomic DNA
Project description:UV-induced CPDs were mapped in primary skin melanocytes or normal human skin fibroblasts following either UVC or UVB irradiation and in isolated human genomic DNA (naked DNA control) that was UVB or UVC irradiated. CPDs were mapped across the human genome using the CPD-capture-seq method and the resulting libraries were captured for ~4000 genomic regions of interest (~3 Mbp) of the human genome by the company Rapid Genomics prior to Illumina sequencing