Project description:Synbiotics are a combination of probiotics and prebiotics which can alter the composition of the gastrointestinal tract evoking beneficial effects throughout the body through the production of a battery of bioactive metabolites. In this study, a synbiotic was used to reduce the behavioral and biochemical symptoms of depression and this nanostring panel was used to decipher where along the gut-brain-axis the synbiotic-derived metabolites were invoking their beneficial effects on the immune system. The synbitoic was composed of two probiotic bacteria (Lactobacillus fermentum ATCC 793 and Bifidobacteria longum ATCC 15707 and a grape -derived prebiotic composed of grape seed polyphenol extract, resveratrol and a concord grape extract. Male mice (C57BL/6) were pretreatment with either nothing (control), BDPP, probiotic or synbiotic and underwent 28 days of chronic unpredicitable stress. After 28 days, animals' behavior reflected an increase in depressive- and anxiety-like behavior, rescued specifically by the synbiotic. This nanostring multiplex analysis reveals both tissue- and treatment-specific effects on immune modulators.
Project description:In this study, the relative effect of consuming five different commercial feeds (Feed B-F) and and frozen fish (control; Feed A) for two months on our selected juvenile Asian seabass (77.3g ± 22.4g) were investigated. The growth performance, the biochemical analysis of their flesh, the histology of their midgut and the transcriptome of their midgut and liver were compared to each other and controls. Our customised agilent microarray platform (GPL17855), were used to investigate the liver transcriptomic expression of the different Groups fed with different Feeds. No differential expression were detected between commerical Feeds (B, C, E and F). A total of 397 differentially expressed transcripts (Foldchange cutoff: -1.5⤠or â¥1.5, Pvalue with FDR (<0.05) were detected between Feed D and control Feed A. Total: 24 samples (4 biological samples per Group; 6 Different Groups in total). Groups (A-F) were fed with Feeds (A-F) respectively for 61 days.
Project description:In this study, the relative effect of consuming five different commercial feeds (Feed B-F) and and frozen fish (control; Feed A) for two months on our selected juvenile Asian seabass (77.3g ± 22.4g) were investigated. The growth performance, the biochemical analysis of their flesh, the histology of their midgut and the transcriptome of their midgut and liver were compared to each other and controls. Our customised agilent microarray platform (GPL17855), were used to investigate the liver transcriptomic expression of the different Groups fed with different Feeds. No differential expression were detected between commerical Feeds (B, C, E and F). A total of 397 differentially expressed transcripts (Foldchange cutoff: -1.5≤ or ≥1.5, Pvalue with FDR (<0.05) were detected between Feed D and control Feed A.
Project description:This study examined gene expression in liver, fat and skeletal muscle of juvenile baboons to determine the effect of maternal under-nutrition (MUN; dams were fed a diet with 30% reduction in nutrients) during pregnancy on response to consumption of a high-fat, high-carbohydrate, high-salt and sugar drink (HFCS) diet for 7 weeks later in development of MUN offspring. After dietary challenge, gene expression in the liver, fat and skeletal muscle of MUN juvenile baboons was compared with that of control juvenile baboons (CON; dams were fed a control diet). Results showed that the number of differentially expressed genes and enriched pathways in these tissues differed between MUN and CON juvenile baboons.