Ontology highlight
ABSTRACT:
PROVIDER: PRJNA735049 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14728973.fastq.gz | Fastqsanger.gz | |||
| SRR14728974.fastq.gz | Fastqsanger.gz | |||
| SRR14728975.fastq.gz | Fastqsanger.gz | |||
| SRR14728976.fastq.gz | Fastqsanger.gz | |||
| SRR14728977.fastq.gz | Fastqsanger.gz |
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Project description:Metabolomics of Arabidopsis photorespiration mutants under fluctuating light conditions
2023-04-26 | MSV000091798 | GNPS
Project description:Metabolomics of Arabidopsis photorespiration mutants under fluctuating light conditions
2023-04-26 | MSV000091798 | MassIVE
Project description:Purpose: Overexpression of VDE, ZEP and PsbS (VPZ) in plants confers enhanced dynamics of non-photochemical quenching of NPQ under FL light. VPZ overexpression in tobacco has been reported to increase plant biomass under fluctuating light. However, our work demonstrates that such strategy had no advantageous effect when asssayed in Arabidopsis thaliana. To investigate whether this observation is due to impairements in signaling pathways due to the expression of VPZ in Arabidopsis thaliana, transcriptome profiles of VPZ plants exposed to fluctuating light or high light were conducted. Methods: mRNA-Seq libraries were prepared from 21-d-old Col0 plants and VPZ lines #2 and #4 cultivated under standard control conditions and then exposed to fluctuating light or high light for 6h and 30 h. Standard Illumina protocols were used for mRNA-Seq sequencing. Triplicates for 3 independent plants were used. Adaptor sequences were removed with Trimmomatic and resulting reads mapped to the Arabidopsis genome (Araport11) with Tophat 2.1.1. Normalised counts reads were quantified with featureCounts to provide processed data files and differential expression analysis were conducted with the DEseq2 pipeline. Results: mRNA-Seq libraries contained app. 9 milion reads each. Transcript analysis for differential gene expression was conducted according to the Tophat/featureCounts/DESeq2 pipeline. To declare significant differences, a cut-off for absolute log2(FC) ≥ 1 compared to the initial timepoint (0h) and FDR ≤ 0.05 was applied. Significant transcripts exclusively found for Col0 and both VPZ lines in each time-point were considered for subsequent analysis. Accordingly, 528 and 415 transcripts (6h and 30h) were found for Col0, and 326 and 896 (6h and 30h) for the VPZ under control conditions. For FL, 490 and 591 transcripts qualified as specific (6h and 30h) for Col0 and 938 and 401 (6h and 30h) for VPZ. Strikingly, the amount of transcripts raised to 2847 and 2902 DEGs (6h and 30h) for Col0, whereas only 546 and 304 (6h and 30h) were detected for VPZ under HL. transcripts). Conclusions: the overexpression of VPZ in Arabidopsis leads to particular transcriptome response under fluctuating light related to changes in photosynthesis and response to abscisic acid, among all. However, Col0 displayed wider transcriptome responses than VPZ after exposure to high light .
2022-09-08 | GSE137650 | GEO
Project description:Plants experience dynamic light daily, with light conditions varying on a second-by-second basis. Little is understood about the mechanisms that allow plants to survive such variable conditions. Here, we have exposed Arabidopsis thaliana plants to naturally fluctuating light regimes alongside traditional square light regimes. The response was highly consistent across experiments, leading us to believe there is an epigenetic mechanism involved. We show significant alterations in DNA methylation between fluctuating light acclimated plants, and square light acclimated plants, demonstrating the frequency of fluctuations impacts the plant methylation. This was accompanied by significant changes in gene expression, some of which correlated with altered DNA methylation. Interestingly, several transposable elements which displayed differential methylation were found to be differentially expressed between light regimes. This data suggests DNA methylation may have a role in acclimation to natural light which may directly regulate gene expression and impact transposable element activation.
2025-09-02 | GSE261531 | GEO
Project description:Plants experience dynamic light daily, with light conditions varying on a second-by-second basis. Little is understood about the mechanisms that allow plants to survive such variable conditions. Here, we have exposed Arabidopsis thaliana plants to naturally fluctuating light regimes alongside traditional square light regimes. The response was highly consistent across experiments, leading us to believe there is an epigenetic mechanism involved. We show significant alterations in DNA methylation between fluctuating light acclimated plants, and square light acclimated plants, demonstrating the frequency of fluctuations impacts the plant methylation. This was accompanied by significant changes in gene expression, some of which correlated with altered DNA methylation. Interestingly, several transposable elements which displayed differential methylation were found to be differentially expressed between light regimes. This data suggests DNA methylation may have a role in acclimation to natural light which may directly regulate gene expression and impact transposable element activation.
2025-09-02 | GSE261532 | GEO
Project description:Increasing photosynthetic efficiency remains a major goal across the globe given its central role in CO2 assimilation. Here, we identified a nuclear genome-residing orphan gene, comprised of 3 exons, two with apparent endophytic origins and the third being a highly conserved fragment of the RIBULOSE BISPHOSPHATE CARBOXYLASE/ OXYGENASE LARGE SUBUNIT (RuBisCo). This novel gene, here-on referred to as Populus RuBisCo-like (PRL-1) gene localizes in chloroplast, endoplasmic reticulum and nuclear compartments of plant cells, acts as a transcriptional repressor and modulates adaptation to fluctuating light. P. trichocarpa genotypes with high PRL-1 expression levels quickly dissipated non-photochemical quenching (NPQ) upon transitioning from high to low light and rapidly induced NPQ upon returning to high light. The dynamic modulation of NPQ yields high quantum efficiency of linear electron transport (PSII) and 10-20% increased quantum efficiency of CO2 assimilation (CO2). The enhanced photosynthesis efficiency corresponded with increased plant height and biomass in P. trichocarpa with up to 35 % and 100% increases under field and greenhouse conditions. Transgenic Arabidopsis plants heterologously expressing the PRL-1 gene gained up to 200% in biomass and 97% in seed yield under greenhouse conditions. Taken together, PRL-1 presents a novel and trackable target for increasing photosynthetic efficiency in plants.
2023-04-24 | MSV000091785 | MassIVE
Project description:Plants in the natural environment experience continuous dynamic changes in light intensity. Here, we exposed Arabidopsis thaliana plants to naturally fluctuating light regimes alongside traditional square light regimes such as those often found in control environment growth chambers. The physiological response was highly consistent across experiments in sibling plants, indicating the possibility of an epigenetic mechanism, leading us to investigated differences in DNA methylation. Our results identified a large number of changes in DNA methylation patterns between fluctuating light acclimated plants and square light acclimated plants, demonstrating natural fluctuations in light impacts plant epigenetic mechanisms. Most importantly, there are more differences in DNA methylation patterns between different light pattern regimes than between different light intensities. These differences in DNA methylation were accompanied by significant changes in gene expression, some of which correlated with altered DNA methylation. One of these genes, MCCA, was found to significantly impact photosynthetic efficiency when knocked out. Thousands of transposable elements copies were differentially methylated between light regimes. Interestingly, up to 30% of these TEs are linked to nearby differentially expressed genes. Our data suggests DNA methylation plays a role in acclimation to natural light which may directly regulate gene expression and impact transposable element activation.
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Project description:GWAS identifies a OsPIF4 gene underlying iWUE under fluctuating light and drought combined stress in rice
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Project description:Transcriptional Response of Arabidopsis thaliana to Fluctuating Light
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