Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Micro-engineered perfusable 3D-bioprinted glioblastoma model for in-vivo mimicry of tumor microenvironment

ABSTRACT: Micro-engineered perfusable 3D-bioprinted glioblastoma model for in-vivo mimicry of tumor microenvironment

PROVIDER: PRJNA738713 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14851323.fastq.gz	Fastqsanger.gz
	SRR14851324.fastq.gz	Fastqsanger.gz
	SRR14851325.fastq.gz	Fastqsanger.gz
	SRR14851326.fastq.gz	Fastqsanger.gz
	SRR14851327.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Micro-engineered perfusable 3D-bioprinted glioblastoma model for in-vivo mimicry of tumor microenvironment

Project description:We aimed to evaluated the effect of the cells growth conditions on the genetic profile of the cells. More specifically, we wanted to evaluate whether our 3D cancer model better reflects the in vivo scenario than 2D culture methods . Therfore, we performed RNA-seq of glioblastoma tumors grown in mice and compared it to a "penta-culture" comprised of GL261 murine GB cells, primary astrocytes, microglia, pericytes, and endothelial cells, grown in 2D or in 3D-bioink

2021-08-18 | GSE178371 | GEO

Versatile human cardiac tissues engineered with perfusable heart extracellular microenvironment for biomedical applications

Project description:Versatile human cardiac tissues engineered with perfusable heart extracellular microenvironment for biomedical applications

| PRJNA966148 | ENA

Versatile human cardiac tissues engineered with perfusable heart extracellular microenvironment for biomedical applications

Project description:Engineered human cardiac tissues have been utilized for various biomedical applications, including drug testing, disease modeling, and regenerative medicine. However, the applications of cardiac tissues derived from human pluripotent stem cells are often limited due to their immaturity and lack of functionality. Therefore, in this study, we established a perfusable culture system based on in vivo-like heart microenvironments to improve human cardiac tissue fabrication. The integrated culture platform of a microfluidic chip and a three-dimensional heart extracellular matrix enhanced human cardiac tissue development and their structural and functional maturation. These tissues were comprised of cardiovascular lineage cells, including cardiomyocytes and cardiac fibroblasts derived from human induced pluripotent stem cells, as well as vascular endothelial cells. The resultant macroscale human cardiac tissues exhibited improved efficacy in drug testing (small molecules with various levels of arrhythmia risk), disease modeling (long QT syndrome and cardiac fibrosis), and regenerative therapy (myocardial infarction treatment). Therefore, our culture system can serve as a highly effective tissue-engineering platform to provide human cardiac tissues for versatile biomedical applications.

2024-02-03 | PXD042077 | Pride

Modeling early human heart development using an iPSC-based 3D bioprinted model of embryonic heart tube

Project description:Human heart development is a precisely coordinated process involving dynamic transcriptional and morphological changes. Due to limited access to the developing human heart in utero and lack of accurate experimental models, the cell-microenvironmental interactions underlying human heart development remain elusive. Advances in 3D bioprinting and stem cell technologies have enabled the fabrication of in vitro analogues of cardiac tissues with complex structures, while incorporating cell intrinsic (genetic) and microenvironment factors. Here we present a perfusable 3D bioprinted model of human embryonic heart tube (eHT), composed of distinct layers of myocardium, cardiac jelly, and endocardium. Human induced pluripotent stem cell-derived cardiomyocytes and endothelial cells were cocultured in the engineered eHTs under dynamic flow. This system demonstrated robust cell viability and cardiac function, including full lumen endothelialization, progressive myocardial compaction, and global tissue contraction. A promoting effect of dynamic flow on cardiac maturation and cell lineage commitment was revealed by single-cell RNA sequencing. The established eHT model offers a novel research-enabling platform to study human heart development, various (congenital) heart disease, and therapeutic interventions.

2026-02-18 | GSE315240 | GEO

Perfusable Biohybrid Designs for Bioprinted Skeletal Muscle Tissue.

Project description: Not available

| S-EPMC11468554 | biostudies-literature

Bioprinted 3D Outer Retina Barrier Uncovers RPE-dependent Choroidal Phenotype in Advanced Macular Degeneration

Project description:We engineered a native-like 3D-oBRB tissue by bioprinting endothelial cells, pericytes, and fibroblasts on the basal side of a biodegradable scaffold and establishing an RPE monolayer on top. To investigate the communication between endothelial cells, fibroblasts, pericytes and retinal pigment epithelial cells, we grew them as either 2D cell cultures or as 3D bioprinted outer blood retinal barrier tissues and harvested cells for single-cell RNAseq after 6 weeks of maturation.

2022-10-21 | GSE214928 | GEO

Single cell RNA-seq of bioprinted human kidney organoid

Project description:These files represent single cell RNA-Seq data generated on a 10x Chromium genomics platform from bioprinted iPSC-derived human kidney organoids differentiated according to our published protocol (Takasato et al., Nature Protocols 2016). The data contains >2000 cells that passed our QC. Data was used to confirm that bioprinted organoids contain a similar cellular composition as standard manually produced organoids - See https://www.biorxiv.org/content/10.1101/505396v1.

2020-09-01 | GSE138835 | GEO

Matrix directs trophoblast differentiation in a bioprinted organoid model of early placental development

Project description:Trophoblast organoids can provide crucial insights into mechanisms of placentation, however their potential is limited by highly variable extracellular matrices unable to reflect in vivo tissues. Here, we present a bioprinted placental organoid model, generated using the first trimester trophoblast cell line, ACH-3P, and a synthetic polyethylene glycol (PEG) matrix. Bioprinted or Matrigel-embedded organoids differentiate spontaneously from cytotrophoblasts into two major subtypes: extravillous trophoblasts (EVTs) and syncytiotrophoblasts (STBs). Bioprinted organoids are driven towards EVT differentiation and show close similarity with early human placenta or primary trophoblast organoids. Inflammation inhibits proliferation and STBs within bioprinted organoids, which aspirin or metformin (0.5 mM) cannot rescue. We reverse the inside-out architecture of ACH-3P organoids by suspension culture with STBs forming on the outer layer of organoids, reflecting placental tissue. Our bioprinted methodology is applicable to trophoblast stem cells. We present a high-throughput, automated, and tuneable trophoblast organoid model that reproducibly mimics the placental microenvironment in health and disease.

2025-07-28 | GSE279994 | GEO

In Vitro Study of Directly Bioprinted Perfusable Vasculature Conduits.

Project description: Not available

| S-EPMC4283831 | biostudies-literature

Perfusable Tissue Bioprinted into a 3D-Printed Tailored Bioreactor System.

Project description: Not available

| S-EPMC10813239 | biostudies-literature