Project description:Gene expression profiles of chicken preadipocytes were constructed using Chicken Genome Arrays to determine the gene expression patterns of preadipocytes derived from two chicken lines divergently selected for abdominal fat content. Oleate was used as an inducer of preadipocyte differentiation, and the different expressed genes between the normal and oleate treated preadipocyte were analyzed.
Project description:3T3-L1 preadipocytes were differentiated into mature adipocytes using adipogenic differentiation media. Total RNA was isolated at D0 (two days post confluency) and D7 of adipocyte differentiation. nAnT-iCAGE sequencing was performed to analyse transcriptional start sites.
Project description:Immortalized mouse brown preadipocytes were differentiated. Conditioned medium was collected and concentrated. Proteins present in the conditioned medium were analyzed by LC-MS/MS
Project description:Transcription profiling by high throughput sequencing of mouse embryonic fibroblasts, terminally differentiated neurons and embryonic stem cells
Project description:Transcription profiling by high throughput sequencing of retinoic acid-differentiated neuronal cells compared to nondifferentiated pluripotent embryonic stem cells
