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ABSTRACT: Microbiota Tcrd-GDL

PROVIDER: PRJNA741071 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14907227_1.fastq.gz	Fastqsanger.gz
	SRR14907227_2.fastq.gz	Fastqsanger.gz
	SRR14907228_1.fastq.gz	Fastqsanger.gz
	SRR14907228_2.fastq.gz	Fastqsanger.gz
	SRR14907229_1.fastq.gz	Fastqsanger.gz

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Project description:Purpose: compare immature B cells in presence or not of γδ T cells in vivo and in vitro. Methods: immature B cells were sorted from spleen of control Tcrd-GDL mice and Tcrd-GDL mice treated with Diphteria toxin (DTx) for depletrion of γδ T cells after eight days and were sequenced for transcriptional (RNA-seq) and epigenetic profile (ATAC-seq). Further, immature B cells were sorted from spleen of Tcdr-H2B mice, cultured alone or in presence of γδ T cells and sequenced for transcriptional profile (RNA-seq). Results/Conclusions: In presence of γδ T cells, immature B cells upregulated genes related to unfolded protein response and mTORC1 signaling

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