Project description:Single-cell RNA sequencing analysis of RUNX1-RUNX1T1(9a) transformed c-kit positive cells with (Kat2a WT) and without Kat2a (Kat2a NULL). Lineage negative bone marrow cells were collected from Kat2a fl/fl Mx1-Cre-/- and Kat2a fl/fl Mx1-Cre +/- animals after pIpC treatment and transduced with RUNX1-RUNX1T1(9a) expressing retrovirus (reported by GFP expression). Cells were injected into irradiated C57BL6 mice and GFP positive c-Kit positive bone marrow cells collected 2 and 4 months after transplantation. Cells were processed for single-cell RNA sequencing library preparation (10X chromium single cell) and next gene sequencing following 10X genomics v2 protocol.
Project description:Live CD45+ cells from the peripheral blood (PB) and synovial tissue (ST) of 3 naive to treatment RA patients were sorted for single cell RNA sequencing (scRNAseq) using 10x Genomics platform. Library preparation was performed using 10X Single Cell 3’ Reagent Kits v2 and libraries were sequenced on the Illumina HiSeq 4000 system to a minimum depth of 50k reads/cell.
Project description:Endometrial biopsies were collected on menstrual cycle day 7 from healthy fertile women age 24-42. Biopsies were processed to a single cell suspension and submitted for single cell sequencing on the 10x platform.
Project description:The study profiles endometrial samples from donors in reproductive age with and without endometriosis collected either during natural cycles or under exogenous hormonal treatment. Samples where profiled either with single-cell RNA sequencing (scRNA-seq) or single-nuclei RNA sequencing (snRNA-seq) using 10x technology.