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Using RNAseq for identifying hepatic genes involved in multi- (F1 generation) and transgenerational (F2 generation) responses to chronic benzo[a]pyrene (BaP) and triclosan (TCS) mixture exposure (F0 generation) in Xenopus tropicalis.

ABSTRACT: Using RNAseq for identifying hepatic genes involved in multi- (F1 generation) and transgenerational (F2 generation) responses to chronic benzo[a]pyrene (BaP) and triclosan (TCS) mixture exposure (F0 generation) in Xenopus tropicalis.

PROVIDER: PRJNA743018 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15014444.fastq.gz	Fastqsanger.gz
	SRR15014445.fastq.gz	Fastqsanger.gz
	SRR15014446.fastq.gz	Fastqsanger.gz
	SRR15014447.fastq.gz	Fastqsanger.gz
	SRR15014448.fastq.gz	Fastqsanger.gz

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Using RNAseq for identifying hepatic genes involved in multi- (F1 generation) and transgenerational (F2 generation) responses to chronic benzo[a]pyrene (BaP) and triclosan (TCS) mixture exposure (F0 generation) in Xenopus tropicalis.

Project description:We investigated the multi- and transgenerational impact of BaP and TCS mixture on Xenopus females following F0 generation exposure from the tadpole to the mature adult stage at a concentration (50 ng.L-1 each). 5 females from F1 and F2 generations by parentale exposure conditions (control vs Mixture) were used for studying liver transcriptome by RNAseq. RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 75bp single reads

2021-10-30 | GSE179257 | GEO

Using RNAseq for identifying hepatic genes involved in multi- (F1 generation) and transgenerational (F2 generation) responses to chronic benzo[a]pyrene exposure (F0 generation) in Xenopus tropicalis

Project description:We investigated the multi- and transgenerational impact of BaP on Xenopus females following F0 generation exposure from the tadpole to the mature adult stage at a concentration (50 ng.L-1). 5 females from F1 and F2 generations by parentale xposure conditions (control vs BaP) were used for studying liver transcriptome by RNAseq. RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 75bp single reads

2021-01-01 | GSE150911 | GEO

Differential DNA methylation following developmental exposure to triclosan (TCS) in 7dpf zebrafish larvae

Project description:There is increasing evidences that toxicant exposure can alter methylation profiles, particularly during embryogenesis when DNA methylation patterns are established. In order to investigate the effects of TCS on DNA methylation and its consequences on gene expression during embryogenesis, we exposed zebrafish eggs during 7 days post fertilisation (dpf) to 50 and 100 µg/L of TCS. Although, global DNA methylation was not affected by TCS exposure, a total of 171 differentially methylated fragments (DMFs) between conditions were identified. The majority of the DMFs were found between the two exposed groups, reflecting distinct triggered responses. Gene ontology analysis revealed that pathways involved in TGF-beta signaling were enriched in larvae exposed to 50 µg/L, while de novo pyrimidine biosynthesis functions were overrepresented in fish exposed to 100 µg/L of TCS. In parallel, gene expression analysis revealed a positive correlation between DNA methylation patterns and gene expression levels for introns, together with significant alterations of the transcription of genes involved in nervous system development, transcriptional factors and histone methyltransferase. Overall our work provides evidence that TCS impacts DNA methylation during zebrafish early embryogenesis and brings clues about the toxicity mechanisms of TCS during this critical period of development.

2019-10-22 | GSE105766 | GEO

Multigenerational Effects of Dietary Benzo[a]pyrene Exposure on Behavior, Gene Expression, and DNA Methylation

Project description:Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), is implicated in many developmental and behavioral adverse outcomes in offspring of exposed parents. Following a dietary preconceptional exposure to BaP in zebrafish, the objective of this study was to compare parental sex-dependent adverse outcomes in F1 and F2 offspring with the transcriptomic and epigenetic changes in eggs, sperm, and 10-hour post fertilization (hpf) embryos. Adult wild-type (5D) zebrafish were fed 708 µg BaP/g diet (measured) at a rate of 1% body weight twice/day (14 µg BaP/g fish/day) for 21 days. Fish were spawned using a crossover design and parental (F0) behavior and reproductive indexes measured. In offspring behavioral effects were measured at 96 hours post fertilization (hpf) in F1 & F2 larvae, and again when F1s were adult. Compared to controls, there was no significant effect of BaP exposure on adult behavior in F0, but locomotor behavior was significantly increased in F1 adults of both sexes. Larval behavior (96 hpf, photomotor response assay) was significantly altered in both the F1 and F2 generations following parental BaP exposure. To assess parental sex-dependent molecular mechanisms, BaP-mediated differential gene expression and DNA methylation changes were measured using RNAseq and RRBS, respectively, on F0 sperm and eggs and the 10 hpf embryos from all four crosses in F1 generation. Embryos resulting from the BaP male and control female cross had the most differentially methylated regions (DMRs) and differentially expressed genes. Some DMRs were associated with genes encoding chromatin modifying enzymes suggesting regulation of chromatin conformation by DNA methylation. Parental dietary BaP exposure caused persistent behavioral changes wherein the male germline contributed most significantly to the multigenerational adverse outcomes.

2022-05-26 | GSE203631 | GEO

Elucidating the Effects of the Environmental Pollutant Benzo[a]pyrene [BaP] on Cardiac Arrhythmogenicity

Project description:Benzo[a]pyrene (BaP) is a product of combustion and is an abundant constituent of wildfire derived smoke and particulate matter 2.5 (PM2.5). Although arrythmias are associated with wildfire, the mechanism remains unclear. We hypothesized that BaP might predispose individuals to arrhythmias and possibly sudden cardiac death. Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) from healthy donors (n=3) were used to investigate the cardiovascular toxicity of BaP. Across a broad concentration range, BaP did not affect iPSC-CM viability, and a 4-day exposure of BaP at 50 M did not affect contractility. After exposure to BaP at 50 M for 5 days, iPSC-CMs were subjected to bulk RNA sequencing, and analysis revealed markers of inflammation were upregulated, and ion channel expression was modulated. Because inflammation is associated with reactive oxygen species (ROS) production, iPSC-CMs exposed to BaP had a concentration-dependent increase in ROS production. Mitotracker fluorescent imaging revealed decreased mitochondrial membrane potentials after iPSC-CMs treatment with 50 μM BaP. Seahorse assays revealed that BaP exposure decreased oxygen consumption rate, basal respiration, and maximal respiration in a dose-dependent manner. Using multiple electrode array (MEA) assays, iPSC-CMs exposed to 50 M BaP for 3 days, had prolonged field potential duration (FPD) and increased beat period (BP). However, co-treatment with N-acetyl cysteine (NAC), a nucleophile that scavenges ROS and preserves glutathione, attenuated the effect of BaP on FPD and BP (p<0.001). In summary, BaP exposure upregulated inflammatory cytokines and increased ROS production by impairing mitochondrial function. BaP had proarrhythmic effects of increased BP and FPD and was abrogated by NAC. Thus, BaP inhalation from wildfire smoke may predispose individuals to QT prolongation, arrhythmia, and sudden cardiac death.

2024-08-25 | GSE244567 | GEO

Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally II

Project description:People are exposed to polycyclic aromatic hydrocarbons, like benzo[a]pyrene (BaP), via inhalation of particulate matter air pollution and ingestion of grilled and smoked foods. Prenatal exposure to BaP destroys germ cells in ovaries, causing earlier onset of ovarian senescence post-natally. Developing testes are affected at higher doses than ovaries. However, it is not known if adverse effects are transmitted to subsequent generations. We orally dosed pregnant female mice (F0) with 0.033, 0.2, or 2 mg/kg-day BaP or vehicle from embryonic day (E) 6.5-11.5 (F1 offspring) or E6.5-15.5 (F2 and F3). Ovarian germ cell and follicle numbers were significantly decreased in F3 females at all doses of BaP; testicular germ cells were not affected. E13.5 germ cell RNA-sequencing revealed significantly increased expression of male-specific genes in female germ cells across generations and BaP doses. Our study demonstrated that F0 BaP exposure partially disrupted sexual identity of female germ cells transgenerationally.

2023-06-07 | GSE213946 | GEO

Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally I

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Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally [RNA-seq]

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Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally [Bisulfite-Seq]

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MicroRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Agilent miRNA array]

Project description:Lack of change in microRNA expression in adult mouse liver following treatment with benzo(a)pyrene (BaP), as detected using Agilent miRNA arrays. We have investigated the effect of exposure to 150 mg/kg benzo(a)pyrene (BaP) for 3 days on mRNA and miRNA expression levels in adult mouse liver. We used Agilent miRNA array platforms to assess effects of BaP exposure on miRNA expression levels. Our results indicate a distinct lack of effect of BaP of miRNA expression, despite widespread changes in mRNA levels. Keywords: Toxicology, miRNA

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