Treadmill training regulates bone metabolism and osteogenic differentiation(miRNA Array)
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ABSTRACT:
PROVIDER: PRJNA743053 | ENA |
REPOSITORIES: ENA
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Project description:MicroRNAs (miRNAs) are important in the process of exercise influencing bone metabolism. The present study aimed to detect and select differentially expressed miRNAs in the bone tissues of mice trained on a treadmill, predict the target genes of these differentially expressed miRNAs and lay a foundation for exploring the effect of treadmill training on bone metabolism through miRNAs. In this experiment, after the mice were trained on a treadmill for 8 weeks, the mechanical properties of mouse femur bone were assessed, and the alkaline phosphatase (ALP) activity and osteocalcin (OCN) protein levels of the bone were assayed. miRNA microarray and reverse transcription-quantitative (RT-q)PCR were performed to select and validate differentially expressed miRNAs in the bone, and the target genes of these miRNAs were predicted with bioinformatics methods. In addition, the differentially expressed miRNAs in the bone tissues were compared with those in mechanically strained osteocytes in vitro.
2021-07-02 | GSE179201 | GEO
Project description:Endurance training is associated with decreased cardiovascular-related morbidity and mortality. Cardiac hypertrophy is an adaptive mechanism of the heart in response to volume and pressure overload. Nonetheless, murine exercise models for cardiac hypertrophy are still under discussion. Using a small-animal treadmill, a forced intensity controlled training model was conducted to characterize cardiac hypertrophy in mice utilizing multimodal analyses. Wild-type male C57BL/6 mice age between 5-8 weeks old were subjected to 8 weeks of forced endurance training using a small-animal treadmill or sedentary age-matched control. Transcriptomic analysis of 8-week training hearts was evaluated using RNA sequencing. RNA sequencing revealed several genes involved in the cell cycle, apoptosis, contractile protein expression, and organ growth that were among the most differentially regulated genes after 8-weeks of exercise.
2025-12-02 | GSE308626 | GEO
Project description:Traumatic spinal cord injury (SCI) often leads to loss of locomotor function. Neuroplasticity of spinal circuitry underlies some functional recovery and therefore represents a therapeutic target to improve locomotor function following SCI. However, the cellular and molecular mechanisms mediating neuroplasticity below the lesion level are not fully understood. The present study performed a gene expression profiling in the rat lumbar spinal cord at 1 and 3 weeks after contusive SCI at T9 compared to control rat that received sham injury (laminectomy). The below-level gene expression profiles were compared with those of animals that were subjected to treadmill locomotor training. Rat lumbar spinal cords were taken for the microarray analysis at 1 and 3 weeks after contusive spinal cord injury at the T9 level. Another group of rats received treadmill locomotor training for 3 weeks, and theirs spinal cords were harvested for the microarray. The changes in gene expression after spinal cord injury were analyzed at the two time points. The influence of treadmill locomotor training was evaluated by comparing gene expression profiles between animals with or without treadmill training.
2013-11-27 | E-GEOD-52763 | biostudies-arrayexpress
Project description:This study investigates the effects of exercise and genetic predisposition on the transcriptomic profile of the pituitary gland of long-term selected marathon (DUhTP) and non-inbred (DUC) mice. In the exercise group, mice underwent treadmill training for three weeks. For the sedentary control group, mice were kept under minimal physical activities. For the 3-week training program, the mice were running five days per week (Monday to Friday) starting at age of 49 days after birth (Walz et. al. 2021). All mice used in this experiment were male and sacrificed at day 70 of life for tissue sampling.
2024-11-24 | E-MTAB-12063 | biostudies-arrayexpress
Project description:This study investigates the effects of exercise and genetic predisposition on the transcriptomic profile of the rectus femoris muscle of long-term selected marathon (DUhTP) and non-inbred (DUC) mice. All mice used in this experiment were male. In the exercise group, mice underwent treadmill training for three weeks. For the sedentary control group, mice were kept under minimal physical activities. For the 3-week training program, the mice were running five days per week (Monday to Friday) starting at age of 49 days after birth (Walz et. al. 2021). All mice were sacrificed at day 70 of life for tissue sampling.
2024-11-24 | E-MTAB-12072 | biostudies-arrayexpress
Project description:Traumatic spinal cord injury (SCI) often leads to loss of locomotor function. Neuroplasticity of spinal circuitry underlies some functional recovery and therefore represents a therapeutic target to improve locomotor function following SCI. However, the cellular and molecular mechanisms mediating neuroplasticity below the lesion level are not fully understood. The present study performed a gene expression profiling in the rat lumbar spinal cord at 1 and 3 weeks after contusive SCI at T9. The below-level gene expression profiles were compared with those of animals that were subjected to treadmill locomotor training.
2013-11-27 | GSE52763 | GEO
Project description:In this study, we proved the exercise-induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through establishing exercise animal model by treadmill running experiment. miRNA sequencing analysis identified 16 upregulated and 2 downregulated miRNAs in exercise group.
2021-06-23 | GSE168712 | GEO
Project description:In this study, we proved the exercise-induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) through establishing exercise animal model by treadmill running experiment. lncRNA sequencing analysis identified 44 upregulated lncRNAs and 39 downregulated lncRNAs in exercise group.
2021-06-23 | GSE168711 | GEO
Project description:This project aims to study the change of brain protein lactylation under treadmill exercise training
2024-06-21 | PXD053340 |
Project description:The goal of the endurance exercise training study in young adult rats was to perform exercise training studies in young adult (6-month-old) F344 rats, and from these rats collect multiple tissues in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia. For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max and training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
2023-09-30 | GSE242355 | GEO