Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of HaCat cells after treatment with siRNA knockdown of Agrin

ABSTRACT: Transcriptome analysis of HaCat cells after treatment with siRNA knockdown of Agrin

PROVIDER: PRJNA743236 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15021571.fastq.gz	Fastqsanger.gz
	SRR15021572.fastq.gz	Fastqsanger.gz
	SRR15021573.fastq.gz	Fastqsanger.gz
	SRR15021574.fastq.gz	Fastqsanger.gz
	SRR15021575.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Transcriptome analysis of HaCat cells after treatment with siRNA knockdown of Agrin

Project description:This work describes the alteration in the expression of genes in response to siRNA knockdown of Agrin.

2021-08-23 | GSE179322 | GEO

Methylated RNA immunoprecipitation sequencing (MeRIP-seq) of human epidermis and HaCAT cells with or without ALKBH5 knockdown

Project description:We performed MeRIP-seq on (1) two human epidermis tissue and HaCAT (human keratinocyte cell line) cells; (2) HaCAT cell lines with or without ALKBH5 knockdown by siRNA in duplicate experiments.

2022-08-18 | GSE211442 | GEO

siRNA-mediated knockdown

Project description:siRNA-mediated knockdown

| PRJNA541912 | ENA

Total RNA-seq of C2C12 myotubes treated with agrin or MuSK antibodies

Project description:Total RNA-seq was performed to investigate if MuSK antibody binding directly affects (NMJ) gene expression in muscle cells. C2C12 differentiated myotubes were treated for 16 hours with bivalent MuSK antibodies (activating MuSK signaling) or functionally monovalent MuSK antibodies (inhibiting agrin-induced MuSK signaling) derived from B-cell receptor sequences from a MuSK myasthenia gravis patient. The total RNA transcriptome was compared to treatment with agrin (the natural activator of MuSK) or a negative control antibody targeting a protein not present in myotubes.

2026-01-20 | GSE316686 | GEO

RNAseq of cultured neonatal rat ventricular cardiomyocytes treated with siRNA for Btg1 and Btg2 knockdown.

Project description:Total RNA was isolated from n=3 culture wells per siRNA treatment group, from 6 independent primary neonatal rat cardiomyocyte isolations. RNA was pooled to obtain n=3 independent samples per siRNA treatment group for sequencing. The siRNA treatment groups were= siScr (Scramble control), siBtg12 (Btg1/Btg2 double knockdown), siBtg1 (Btg1 single knockdown), siBtg2 (Btg2 single knockdown).

2023-05-24 | GSE203493 | GEO

Protein dataset of immortalized HaCaT cells p53 knock down HaCaT keratinocytes

Project description:The p53 protein is encoded by TP53 gene and plays the key role in significant number of cellular processes including proliferation, apoptosis and regulation of many stress response pathways. P53 acts like a direct transcription activator of numerous genes regulating cell cycle arrest, DNA repair, growth inhibition and many others (Mollereau and Ma, 2014). The canonical biological function of p53 is maintaining genome integrity via elimination of damaged or exposed to genotoxic stress cells. Immortalized HaCaT cells are widely used for keratinocyte research, since they maintain stable keratinocyte phenotype, have nearly unlimited proliferative potential, do not require specific growth and differentiation factors (Colombo et al., 2017). Also, HaCaT cells produce typical differentiation markers such as cytokeratins K14 and K10, involucrin (Colombo et al., 2017) and respond to keratinocyte differentiation stimuli. Taking together, HaCaT cells have similar to normal human keratinocytes (NHK) properties, however, as many of spontaneously immortalized cell lines HaCaT cells bear two mutant p53 alleles - R282Q and H179Y (Lehman et al., 1993). Mutp53 in HaCaT has an increased affinity to other p53 family members (p63, p73), which significantly expands p53 properties. Moreover, mutp53 indirectly affects specific target genes via protein-protein interactions with other transcription factors (NF-Y, E2F1, NF-KB) or by tethering p63 to new promotor locations. For more detailed investigation of mutp53 impact on various processes in HaCaT cells we performed a shRNA mediated knockdown of mutp53. For generation of stable TP53 knockdown we employed plasmid vector pLKO-p53-shRNA-941 (Addgene # #25637) followed by puromycin selection of transduced cells. Here we present proteomic dataset obtained from wild type HaCaT cells and p53 knock down HaCaT keratinocytes.

2022-04-28 | PXD033538 | Pride

Tyrosine phosphorylation modification omics of HCC38 cells following knockdown of WSTF with siRNA

Project description:Tyrosine phosphorylation modification omics of HCC38 cells following knockdown of WSTF with siRNA

2025-05-29 | PXD064404 |

MLX siRNA knockdown in primary human hepatocytes

Project description:MLX siRNA knockdown in primary human hepatocytes

| PRJNA907289 | ENA

siRNA knockdown in neonatal rat cardiac myocytes

Project description:siRNA knockdown in neonatal rat cardiac myocytes

| PRJNA499086 | ENA

ENO1 siRNA knockdown in Human HeLa cells

Project description:ENO1 siRNA knockdown in Human HeLa cells