Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Plasmodium falciparum

ABSTRACT: Plasmodium falciparum Targeted Locus (Loci)

PROVIDER: PRJNA74597 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR388395.fastq.gz	Fastqsanger.gz
	SRR388396.fastq.gz	Fastqsanger.gz
	SRR388397.fastq.gz	Fastqsanger.gz
	SRR388398.fastq.gz	Fastqsanger.gz
	SRR388399.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 208

Similar Datasets

Plasmodium falciparum

Project description:Plasmodium falciparum Targeted Locus (Loci)

| PRJNA290960 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum Targeted Locus (Loci)

| PRJNA630847 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum Targeted Locus (Loci)

| PRJNA1287770 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum targeted sequencing

| PRJNA454490 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum targeted sequencing of genome-wide loci in Ethiopia

| PRJNA978031 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum targeted sequencing of genome-wide loci in Zambia

| PRJNA862735 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum Targeted genome sequencing

| PRJNA1170993 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum targeted genome sequencing

| PRJNA1171010 | ENA

Plasmodium falciparum

Project description:Plasmodium falciparum Targeted genome sequencing

| PRJNA1129133 | ENA

Heterochromatin de novo formation and maintenance in Plasmodium falciparum

Project description:In the malaria parasite Plasmodium falciparum, the expression of many genes is regulated by heterochromatin (HC) based on the histone mark tri-methylation of histone H3 lysine 9 (H3K9me3). HC assembly involves three distinct steps: de novo nucleation, spreading and maintenance. Nucleation, which consists in formation of HC in a previously euchromatic region, determines at which specific regions of the genome HC occurs, but this process is not well understood in malaria parasites. Here we investigated the DNA sequence cis determinants of HC nucleation in P. falciparum, using a screening approach based on integration of fragments from different heterochromatic genes into an euchromatic locus, followed by chromatin immunoprecipitation (ChIP). We found that fragments of var gene upstream regions nucleated HC efficiently, whereas fragments from the pfap2-g upstream region or from the mspdbl2 locus did not nucleate HC. Fragments from the beginning of the coding sequence (CDS) of pfap2-g nucleated HC with low efficiency, as evidenced by nucleation requiring long fragments of ~2 kb and occurring only in a fraction of the parasites. These results demonstrate that the primary DNA sequence is a main determinant of HC nucleation in P. falciparum. We also studied HC maintenance at the pfap2-g locus, which demonstrated that specific parts of the upstream region, different from the regions competent for HC nucleation, are required for maintenance. Together, our results provide initial insight into how HC is directed to specific loci and maintained in P. falciparum.

2025-04-07 | GSE287562 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data