Project description:DNA methylation profiling of human FFPE meningioma samples These samples were processed as part of a study developing and validating a targeted gene expression biomarker of meningioma outcomes and benefit from radiotherapy.
Project description:DNA methylation from human meningioma samples that were also profiled for spatial heterogeneity analysis. Some samples represent spatially distinct regions, punched using a 2mm core punch from FFPE blocks in a given tumor. Other samples represent serial tumor samples at index treatment and then recurrence.
Project description:Tumor development relies on numerous factors, including the capacity to adapt to hypoxic conditions. Hypoxic microenvironment is a critical driver of the malignant phenotype, which is often correlated with meningioma grade, aggressive behavior, therapeutic resistance, and higher recurrence rates, leading to poor prognosis. Epigenetic alterations are key regulators of gene expression. Changes in DNA methylation patterns are the most extensively characterized epigenetic modifications associated with tumorigenesis. Metastasis, severity, and recurrence of meningioma have all been associated with hypo- and hypermethylation of various genes. We aim to identify the hypoxia-induced genome-wide DNA methylation profile of meningioma.
