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Sequencing of siRNA from soybean containing silencing vectors and empty vectors

ABSTRACT: Sequencing of siRNA from soybean containing silencing vectors and empty vectors

PROVIDER: PRJNA746614 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15142504.fastq.gz	Fastqsanger.gz
	SRR15142505.fastq.gz	Fastqsanger.gz
	SRR15142506.fastq.gz	Fastqsanger.gz
	SRR15142507.fastq.gz	Fastqsanger.gz
	SRR15142508.fastq.gz	Fastqsanger.gz

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Sequencing of siRNA from soybean containing silencing vectors and empty vectors

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Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing.

Project description:BackgroundRNA interference (RNAi) was originally identified as a biological process in which short double-stranded RNA (dsRNA) suppress the expression of genes complimentary to the dsRNA. This cellular intrinsic gene silencing mechanism has subsequently been developed as a useful tool for studies of gene function. A major strategy for producing small interfering RNA (siRNA) in cultured cells involves the use of siRNA expression vectors in which a RNA polymerase III (Pol III) promoter and transcription stop signal are designed to constitute a functional siRNA expression cassette for production of siRNA. However, most of the available vectors contain only one siRNA expression cassette.ResultsIn order to maximize the efficiency and versatility of the vector-based siRNA approach, we have developed vectors containing multiple (up to six) tandem siRNA expression cassettes. Moreover, we demonstrated that these vectors can be used not only to produce different siRNA to simultaneously suppress the expression of multiple genes but also to maximize the silencing of a single gene.ConclusionThe vectors containing multiple siRNA expression cassettes can serve as useful tools for maximizing the efficiency of gene silencing.

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Expression data of cultured MCF10A cells transduced with MYB or MYB-NFIB expression vectors

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