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Single cell RNA sequencing profile of progeny of a unique mesenchymal progenitor subset traced to adult mouse skeletal muscle

ABSTRACT: Single cell RNA sequencing profile of progeny of a unique mesenchymal progenitor subset traced to adult mouse skeletal muscle

PROVIDER: PRJNA748087 | ENA |

REPOSITORIES: ENA

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Single cell RNA sequencing profile of progeny of a unique mesenchymal progenitor subset traced to adult mouse skeletal muscle

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Single-cell RNA sequencing analysis of non-haematopoietic DNGR-1 lineage traced cells from murine spinal cord

Project description:Stem cells are defined by two cardinal properties: limitless self-renewal and multipotency. We have serendipitously found that non-haematopoietic DNGR-1 lineage traced cells residing in the ependymal cell layer of the central nervous system display the two cardinal properties of stem cells, both in vitro and in vivo. However, whether these properties were a feature of all DNGR-1-traced cells or were confined to a particular subset of these is unclear. To address the potential heterogeneity of DNGR-1-traced ependymal cells and caractherise their putative stem cell compartment we conducted single-cell RNA sequencing of DNGR-1-traced cells isolated from uninjured spinal cords.

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